Composition of the synaptic PSD-95 complex.

Composition of the synaptic PSD-95 complex. Research Abstract Details 

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Composition of the synaptic PSD-95 complex. Abstract Text:

Ayse Dosemeci,Anthony J Makusky,Ewa Jankowska-Stephens,Xiaoyu Yang,Douglas J Slotta,Sanford P Markey,

Postsynaptic density protein 95 (PSD-95), a specialized scaffold protein with multiple protein interaction domains, forms the backbone of an extensive postsynaptic protein complex that organizes receptors and signal transduction molecules at the synaptic contact zone. Large, detergent-insoluble PSD-95-based postsynaptic complexes can be affinity-purified from conventional PSD fractions using magnetic beads coated with a PSD-95 antibody. In the present study purified PSD-95 complexes were analyzed by LC/MS/MS. A semiquantitative measure of the relative abundances of proteins in the purified PSD-95 complexes and the parent PSD fraction was estimated based on the cumulative ion current intensities of corresponding peptides. The affinity-purified preparation was largely depleted of presynaptic proteins, spectrin, intermediate filaments, and other contaminants prominent in the parent PSD fraction. We identified 525 of the proteins previously reported in parent PSD fractions, but only 288 of these were detected after affinity purification. We discuss 26 proteins that are major components in the PSD-95 complex based upon abundance ranking and affinity co-purification with PSD-95. This subset represents a minimal list of constituent proteins of the PSD-95 complex and includes, in addition to the specialized scaffolds and N-methyl-d-aspartate (NMDA) receptors, an abundance of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors, small G-protein regulators, cell adhesion molecules, and hypothetical proteins. The identification of two Arf regulators, BRAG1 and BRAG2b, as co-purifying components of the complex implies pivotal functions in spine plasticity such as the reorganization of the actin cytoskeleton and insertion and retrieval of proteins to and from the plasma membrane. Another co-purifying protein (Q8BZM2) with two sterile alpha motif domains may represent a novel structural core element of the PSD.

Composition of the synaptic PSD-95 complex. Publishing Authors By Initials

A Dosemeci,AJ Makusky,E Jankowska-Stephens,X Yang,DJ Slotta,SP Markey,

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Composition of the synaptic PSD-95 complex. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Intr

Journal: Molecular & cellular proteomics : MCP

VOLUME: 6

Page Numbers: 1749-60

Journal Abbreviation: Mol. Cell Proteomics

ISSN: 1535-9476

DAY: 9

MONTH: 07

YEAR: 2007

Composition of the synaptic PSD-95 complex. Information

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LANGUAGE: eng

NlmUniqueID: 101125647

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Grant and Affiliation Information for Composition of the synaptic PSD-95 complex.

AFFILIATION: Laboratory of Neurobiology, NINDS, National Institutes of Health, Bethesda, Maryland 20892, USA.

Country: United States

AGENCY: United States NIMH

GRANT: Z01 MH000279-25

ACRONYM: MH

MEDLINETA: Mol Cell Proteomics

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