Complex spatial distribution and dynamics of an abundant Escherichia coli outer membrane protein, LamB.

Complex spatial distribution and dynamics of an abundant Escherichia coli outer membrane protein, LamB. Research Abstract Details 

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Complex spatial distribution and dynamics of an abundant Escherichia coli outer membrane protein, LamB. Abstract Text:

Karine A Gibbs,Daniel D Isaac,Jun Xu,Roger W Hendrix,Thomas J Silhavy,Julie A Theriot,

Advanced techniques for observing protein localization in live bacteria show that the distributions are dynamic. For technical reasons, most such techniques have not been applied to outer membrane proteins in Gram-negative bacteria. We have developed two novel live-cell imaging techniques to observe the surface distribution of LamB, an abundant integral outer membrane protein in Escherichia coli responsible for maltose uptake and for attachment of bacteriophage lambda. Using fluorescently labelled bacteriophage lambda tails, we quantitatively described the spatial distribution and dynamic movement of LamB in the outer membrane. LamB accumulated in spiral patterns. The distribution depended on cell length and changed rapidly. The majority of the protein diffused along spirals extending across the cell body. Tracking single particles, we found that there are two populations of LamB--one shows very restricted diffusion and the other shows greater mobility. The presence of two populations recalls the partitioning of eukaryotic membrane proteins between 'mobile' and 'immobile' populations. In this study, we have demonstrated that LamB moves along the bacterial surface and that these movements are restricted by an underlying dynamic spiral pattern.

Complex spatial distribution and dynamics of an abundant Escherichia coli outer membrane protein, LamB. Publishing Authors By Initials

KA Gibbs,DD Isaac,J Xu,RW Hendrix,TJ Silhavy,JA Theriot,

For similar proteins: membrane proteins: receptors, cell surface: receptors, virus research abstracts see: proteins: membrane proteins: receptors, cell surface: receptors, virus research

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Complex spatial distribution and dynamics of an abundant Escherichia coli outer membrane protein, LamB. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Molecular microbiology

VOLUME: 53

Page Numbers: 1771-83

Journal Abbreviation: Mol. Microbiol.

ISSN: 0950-382X

DAY: 27

MONTH: Sep

YEAR: 2004

Complex spatial distribution and dynamics of an abundant Escherichia coli outer membrane protein, LamB. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8712028

Complex spatial distribution and dynamics of an abundant Escherichia coli outer membrane protein, LamB. Keywords Mesh Terms:

KEYWORDS: Receptors, Virus

MESH TERMS: metabolism

Chemical & Substance for Abstract: Complex spatial distribution and dynamics of an abundant Escherichia coli outer membrane protein, LamB. Information

Substance Name: maltoporins

Registry Number: 0

Grant and Affiliation Information for Complex spatial distribution and dynamics of an abundant Escherichia coli outer membrane protein, LamB.

AFFILIATION: Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA.

Country: England

AGENCY: United States NIGMS

GRANT: GM47795

ACRONYM: GM

MEDLINETA: Mol Microbiol

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