Complete purification of phosphatidylinositol-specific phospholipase C from a strain of Bacillus thuringiensis.

Complete purification of phosphatidylinositol-specific phospholipase C from a strain of Bacillus thuringiensis. Research Abstract Details 

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Complete purification of phosphatidylinositol-specific phospholipase C from a strain of Bacillus thuringiensis. Abstract Text:

H Ikezawa,T Nakabayashi,K Suzuki,M Nakajima,T Taguchi,R Taguchi,

A phosphatidylinositol-specific phospholipase C was purified from the culture broth of Bacillus thuringiensis IAM 12077 to a homogeneous state as revealed by polyacrylamide gel electrophoresis. The specific activity of the purified enzyme was 559 units/mg and recovery of the enzyme activity was 31%. Molecular and physiological properties of the purified enzyme, including molecular weight (22,000), isoelectric point (pI = 4.9) and its ectoenzyme-releasing activity, were studied in comparison with those other known enzymes of bacterial origin.

Complete purification of phosphatidylinositol-specific phospholipase C from a strain of Bacillus thuringiensis. Publishing Authors By Initials

H Ikezawa,T Nakabayashi,K Suzuki,M Nakajima,T Taguchi,R Taguchi,

For similar type c phospholipases research abstracts see: type c phospholipases research

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Complete purification of phosphatidylinositol-specific phospholipase C from a strain of Bacillus thuringiensis. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 93

Page Numbers: 1717-9

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jun

YEAR: 1983

Complete purification of phosphatidylinositol-specific phospholipase C from a strain of Bacillus thuringiensis. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Complete purification of phosphatidylinositol-specific phospholipase C from a strain of Bacillus thuringiensis. Keywords Mesh Terms:

KEYWORDS: Type C Phospholipases

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: Complete purification of phosphatidylinositol-specific phospholipase C from a strain of Bacillus thuringiensis. Information

Substance Name: Type C Phospholipases

Registry Number: EC 3.1.4.-

Grant and Affiliation Information for Complete purification of phosphatidylinositol-specific phospholipase C from a strain of Bacillus thuringiensis.

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Country: JAPAN

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MEDLINETA: J Biochem

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