Complete deficiency of 5'-nucleotidase activity in Escherichia coli leads to loss of growth on purine nucleotides but not of their excretion.

Complete deficiency of 5'-nucleotidase activity in Escherichia coli leads to loss of growth on purine nucleotides but not of their excretion. Research Abstract Details 

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Complete deficiency of 5'-nucleotidase activity in Escherichia coli leads to loss of growth on purine nucleotides but not of their excretion. Abstract Text:

Masahiro Kakehi,Yoshihiro Usuda,Yukiko Tabira,Shinichi Sugimoto,

Escherichia coli has many periplasmic phosphatase activities. To test whether it can take up and excrete purine nucleotides, we attempted to completely disrupt periplasmic 5'-nucleotidase activity. A 5'-nucleotidase activity was induced in ushA knockout mutant cells, which lack major 5'-nucleotidase activity, when they were grown with purine nucleotides as the sole carbon source. Using DNA macroarrays to compare global gene expression in wild-type and ushA knockout mutant cells cultured with IMP or GMP as the sole carbon source, we identified two genes that were induced in the ushA knockout mutant cells and encoded signal sequence needed for secretion. One of the genes, aphA, encoded a 5'-nucleotidase activity and was induced by IMP or inosine. An ushA aphA double knockout mutant was shown to be unable to grow on purine nucleotides as the sole carbon source. To investigate the excretion of purine nucleotides, we constructed an ushAaphA double knockout mutant of an inosine-producing strain and found that it accumulated IMP in the medium. In addition, when the guaBA operon was introduced into the ushAaphA double knockout IMP producer, GMP was released into the medium. These observations imply the existence of efflux activity for purine nucleotides in E. coli.

Complete deficiency of 5'-nucleotidase activity in Escherichia coli leads to loss of growth on purine nucleotides but not of their excretion. Publishing Authors By Initials

M Kakehi,Y Usuda,Y Tabira,S Sugimoto,

For similar heterocyclic compounds: heterocyclic compounds, 2-ring: purines: purine nucleotides research abstracts see: heterocyclic compounds: heterocyclic compounds, 2-ring: purines: purine nucleotides research

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Complete deficiency of 5'-nucleotidase activity in Escherichia coli leads to loss of growth on purine nucleotides but not of their excretion. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of molecular microbiology and biotechnolog

VOLUME: 13

Page Numbers: 96-104

Journal Abbreviation: J. Mol. Microbiol. Biotechnol.

ISSN: 1464-1801

DAY: 27

MONTH: 11

YEAR: 2007

Complete deficiency of 5'-nucleotidase activity in Escherichia coli leads to loss of growth on purine nucleotides but not of their excretion. Information

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LANGUAGE: eng

NlmUniqueID: 100892561

Complete deficiency of 5'-nucleotidase activity in Escherichia coli leads to loss of growth on purine nucleotides but not of their excretion. Keywords Mesh Terms:

KEYWORDS: Purine Nucleotides

MESH TERMS: secretion

Chemical & Substance for Abstract: Complete deficiency of 5'-nucleotidase activity in Escherichia coli leads to loss of growth on purine nucleotides but not of their excretion. Information

Substance Name: Phosphoric Diester Hydrolases

Registry Number: EC 3.1.4.-

Grant and Affiliation Information for Complete deficiency of 5'-nucleotidase activity in Escherichia coli leads to loss of growth on purine nucleotides but not of their excretion.

AFFILIATION: Fermentation and Biotechnology Laboratories, Ajinomoto Co., Kawasaki, Japan.

Country: Switzerland

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MEDLINETA: J Mol Microbiol Biotechnol

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