[Comparison of two polysaccharide antigens for the serological diagnosis by ELISA of porcine pleuropneumonia (serotype 5)]

[Comparison of two polysaccharide antigens for the serological diagnosis by ELISA of porcine pleuropneumonia (serotype 5)] Research Abstract Details 

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[Comparison of two polysaccharide antigens for the serological diagnosis by ELISA of porcine pleuropneumonia (serotype 5)] Abstract Text:

R Bada Alambedji,J D Dubreuil,

Two antigens of polysaccharidic nature were evaluated for their specificity in serodiagnosis of porcine pleuropneumonia (serotype 5). Recently, long chains lipopolysaccharides (LC-LPS) was shown to increase the specificity of the ELISA test for A. pleuropneumoniae serotype 5 compared to a saline extract of boiled-formalinized whole cells. Nevertheless, some cross reactions were noticed with LC-LPS. We have thus cleaved the LPS molecule at the ketosidic bond between the lipid A and the core. The lipid A was separated from the polysaccharide (PS) by centrifugation. With an indirect sandwich ELISA, it was possible to demonstrate that the PS yielded positive responses with homologous sera (serotype 5) and negative responses with heterologous sera (serotype 3 and undeterminated serotype). Overall, the use of the PS did not notably increase the specificity of the ELISA test. Since production of PS from LC-LPS and its separation from the lipid A is not simple, we do not recommend its use in serodiagnosis.

[Comparison of two polysaccharide antigens for the serological diagnosis by ELISA of porcine pleuropneumonia (serotype 5)] Publishing Authors By Initials

R Bada Alambedji,JD Dubreuil,

For similar animal diseases: swine diseases research abstracts see: animal diseases: swine diseases research

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[Comparison of two polysaccharide antigens for the serological diagnosis by ELISA of porcine pleuropneumonia (serotype 5)] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Zentralblatt für Veterinärmedizin. Reihe B. Journa

VOLUME: 40

Page Numbers: 253-60

Journal Abbreviation: Zentralblatt Veterinarmedizin

ISSN: 0514-7166

DAY: 13

MONTH: Jun

YEAR: 1993

[Comparison of two polysaccharide antigens for the serological diagnosis by ELISA of porcine pleuropneumonia (serotype 5)] Information

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LANGUAGE: fre

NlmUniqueID: 331325

[Comparison of two polysaccharide antigens for the serological diagnosis by ELISA of porcine pleuropneumonia (serotype 5)] Keywords Mesh Terms:

KEYWORDS: Swine Diseases

MESH TERMS: diagnosis

Chemical & Substance for Abstract: [Comparison of two polysaccharide antigens for the serological diagnosis by ELISA of porcine pleuropneumonia (serotype 5)] Information

Substance Name: Antibodies, Bacterial

Registry Number: 0

Grant and Affiliation Information for [Comparison of two polysaccharide antigens for the serological diagnosis by ELISA of porcine pleuropneumonia (serotype 5)]

AFFILIATION: Groupe de Recherche sur les Maladies Infectieuses du Porc (GREMIP), Faculté de médecine vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec, Canada.

Country: GERMANY

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MEDLINETA: Zentralbl Veterinarmed B

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