Comparison of target labeling methods for use with Affymetrix GeneChips.

Comparison of target labeling methods for use with Affymetrix GeneChips. Research Abstract Details 

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Comparison of target labeling methods for use with Affymetrix GeneChips. Abstract Text:

William Lonergan,Toni Whistler,Suzanne D Vernon,William Lonergan,Toni Whistler,Suzanne D Vernon,

BACKGROUND: Several different commercial one-cycle labeling kits are available for preparation of the target for use with the Affymetrix GeneChip platform. However, there have been no evaluations of these different kits to determine if comparable results were generated. We report on the cRNA target synthesis, labeling efficiency and hybridization results using the One-Cycle Target Labeling Assay (Affymetrix), the BioArray RNA Amplification and Labeling System (Enzo Life Sciences), and the Superscript RNA Amplification System (Invitrogen Life Technologies). RESULTS: The only notable difference between kits was in the yield of cRNA target synthesized during in vitro transcription, where the BioArray assay had to be repeated several times in order to have sufficient target. However, each kit resulted in comparable signal and detection calls when hybridized to the Affymetrix GeneChip. CONCLUSION: These 3 one-cycle labeling kits produce comparable hybridization results. This provides users with several kit options and flexibility when using the Affymetrix system.

Comparison of target labeling methods for use with Affymetrix GeneChips. Publishing Authors By Initials

W Lonergan,T Whistler,SD Vernon,W Lonergan,T Whistler,SD Vernon,

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Comparison of target labeling methods for use with Affymetrix GeneChips. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: BMC biotechnology

VOLUME: 7

Page Numbers: 24

Journal Abbreviation: BMC Biotechnol.

ISSN: 1472-6750

DAY: 18

MONTH: 05

YEAR: 2007

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LANGUAGE: eng

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AFFILIATION: Chronic Viral Diseases Branch, Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA. wlonergan@cdc.gov <wlonergan@cdc.gov>

Country: England

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MEDLINETA: BMC Biotechnol

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