Comparison of real-time polymerase chain reaction with the COBAS Amplicor test for quantitation of hepatitis B virus DNA in serum samples.

Comparison of real-time polymerase chain reaction with the COBAS Amplicor test for quantitation of hepatitis B virus DNA in serum samples. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Comparison of real-time polymerase chain reaction with the COBAS Amplicor test for quantitation of hepatitis B virus DNA in serum samples. Abstract Text:

Ming Shi,Yong Zhang,Ying-Hua Zhu,Jing Zhang,Wei-Jia Xu,Ming Shi,Yong Zhang,Ying-Hua Zhu,Jing Zhang,Wei-Jia Xu,

AIM: To compare the clinical performance of a real-time PCR assay with the COBAS Amplicor Hepatitis B Virus (HBV) Monitor test for quantitation of HBV DNA in serum samples. METHODS: The reference sera of the Chinese National Institute for the Control of Pharmaceutical and Biological Products and the National Center for Clinical Laboratories of China, and 158 clinical serum samples were used in this study. The linearity, accuracy, reproducibility, assay time, and costs of the real-time PCR were evaluated and compared with those of the Cobas Amplicor test. RESULTS: The intra-assay and inter-assay variations of the real-time PCR ranged from 0.3% to 3.8% and 1.4% to 8.1%, respectively. The HBV DNA levels measured by the real-time PCR correlated very well with those obtained with the COBAS Amplicor test (r = 0.948). The real-time PCR HBV DNA kit was much cheaper and had a wider dynamic range. CONCLUSION: The real-time PCR assay is an excellent tool for monitoring of HBV DNA levels in patients with chronic hepatitis B.

Comparison of real-time polymerase chain reaction with the COBAS Amplicor test for quantitation of hepatitis B virus DNA in serum samples. Publishing Authors By Initials

M Shi,Y Zhang,YH Zhu,J Zhang,WJ Xu,M Shi,Y Zhang,YH Zhu,J Zhang,WJ Xu,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Comparison of real-time polymerase chain reaction with the COBAS Amplicor test for quantitation of hepatitis B virus DNA in serum samples. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: World journal of gastroenterology : WJG

VOLUME: 14

Page Numbers: 479-83

Journal Abbreviation: World J. Gastroenterol.

ISSN: 1007-9327

DAY: 21

MONTH: Jan

YEAR: 2008

Comparison of real-time polymerase chain reaction with the COBAS Amplicor test for quantitation of hepatitis B virus DNA in serum samples. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100883448

Comparison of real-time polymerase chain reaction with the COBAS Amplicor test for quantitation of hepatitis B virus DNA in serum samples. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Comparison of real-time polymerase chain reaction with the COBAS Amplicor test for quantitation of hepatitis B virus DNA in serum samples. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Comparison of real-time polymerase chain reaction with the COBAS Amplicor test for quantitation of hepatitis B virus DNA in serum samples.

AFFILIATION: Laboratory Center, No. 6 Hospital of Dalian, 1 Chunhai Street, Dalian 116001, Liaoning Province, China. zhsh6655@sohu.com.

Country: China

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: World J Gastroenterol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Comparison of real-time polymerase chain reaction with the COBAS Amplicor test for quantitation of hepatitis B virus DNA in serum samples Related Publications

• Effects of selenium and iodine on the expression of c-fos and c-jun mRNA and their proteins in cultured rat hippocampus neurons.
• Expression of caltrin in the baculovirus system and its purification in high yield and purity by cobalt (II) affinity chromatography.
• Recombinant human interleukin-10 inhibits proliferation of vascular smooth muscle cells stimulated by advanced glycation end products and neointima hyperplasia after carotid injury in the rat.
• [Analysis of the mutations of BRCA1 in 9 familiar breast cancer patients]
• [Application of the interaction models between the polymorphism(s) of metabolic gene(s) and environmental exposure]
• [Arginine vasopressin-induced nitric oxide content changes in cultured cardiac fibroblasts and its relation to nuclear factor-kappaB]
• [Changes in the activity of platelet L-arginine/nitric oxide pathway in hypercholesterolemia patients]
• [Clinical significance of changes in insulin resistance and secretion in multiple organ dysfunction syndrome patients complicated by trauma and hemorrhage]
• [Cloning and sequencing of the genes coding for the histidine-rich protein II of Plasmodium falciparum]
• [Contrast agents in magnetic resonance imaging: development and problems]
• [Differentially expressed genes associated with cold acclimation]
• [Effects of embryonic neural stem cells and glial cell line-derived neurotrophic factor in the repair of spinal cord injury]
• [Effects of ulinastatin on interleukin-8 during one-lung ventilation in surgery]
• [Isolation and cultivation of neural stem cells from the embryonic rat brain and spinal cord]
• [Mechanism of modulation of synaptic efficacy by stress and learning]
• [Opinion on hydatidosis control by immuno-prophylaxis]
• [Oral mucosa graft combined with a local turn-over flap for urethral reconstruction in various hypospadias]
• [Pharmacognostical studies on the roots of Euphorbia prolifera]
• [Pharmacological comparison between virosecurinine and securinine]
• [Phenotyping and genotyping studies in a family with the compound heterozygosity for a deletional delta beta-thalassemia and a beta-thalassemia]
• [Properties of whole-cell potassium currents in mechanically dissociated Drosophila larval central neurons]
• [Rapid diagnosis of non-deletion alpha-thalassemias by reverse dot blot]
• [Retrospective analysis of effectiveness of tonsillar squamous cell carcinoma: report of 108 cases]
• [Significance of hRad17 mRNA expression in human gastric cancer]
• [Study of functional L1 retrotransposon in human type 2 diabetes susceptibility loci]
• [Study on diarrhea disease and Escherichia coli strains harboring HPI pathogenicity island of Yersinia enterolitica in Shandong province]
• [The experimental study of transfected sFlt-Ig gene on K562 leukemia cell growth in nude mice]
• [The influence of postburn administration of rhGH on the host inflammatory response]
• [The transplantation of latissimus dorsi flap of the base of T shape artery with the pedicle of the thoracodorsal artery]
• [Tissue-specific expression of Na(+)-H(+) exchanger isoforms at two developmental stages of human fetus]