Comparison of monolayer and bilayer plates used in antibiotic assay.

Comparison of monolayer and bilayer plates used in antibiotic assay. Research Abstract Details 

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Comparison of monolayer and bilayer plates used in antibiotic assay. Abstract Text:

Standard curves of 5 antibiotics were determined in an antibiotic assay using bilayer and monolayer agar plates and AOAC-specified test organisms and agar media. Micrococcus luteus ATCC 9341a and antibiotic medium No. 2 were used to prepare the penicillin G standard curve. The same organism and antibiotic medium No. 11 were used to prepare the erythromycin standard curve. Standard curves for streptomycin, tetracycline, and gentamicin were prepared, respectively, with antibiotic medium No. 5 and Bacillus subtilis ATCC 6633, antibiotic medium No. 8 and B. cereus ATCC 11778, and antibiotic medium No. 11 and Staphylococcus epidermidis ATCC 12228. Assays of inhibition by meat fortified with penicillin, streptomycin, gentamicin, tetracycline, erythromycin also were performed on monolayer and bilayer plates. Differences in standard curves and inhibitory responses obtained with monolayer and bilayer plates were < 10%. Thus, monolayer plates are acceptable for use in analyses of meat and poultry for antibiotics residues, with savings in laboratory resources and time.

Comparison of monolayer and bilayer plates used in antibiotic assay. Publishing Authors By Initials

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Comparison of monolayer and bilayer plates used in antibiotic assay. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of AOAC International

VOLUME: 81

Page Numbers: 398-402

Journal Abbreviation: J AOAC Int

ISSN: 1060-3271

DAY: 17

MONTH: 03

YEAR: 2008

Comparison of monolayer and bilayer plates used in antibiotic assay. Information

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LANGUAGE: eng

NlmUniqueID: 9215446

Comparison of monolayer and bilayer plates used in antibiotic assay. Keywords Mesh Terms:

KEYWORDS: Reference Standards

MESH TERMS: chemistry

Chemical & Substance for Abstract: Comparison of monolayer and bilayer plates used in antibiotic assay. Information

Substance Name: Culture Media

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Grant and Affiliation Information for Comparison of monolayer and bilayer plates used in antibiotic assay.

AFFILIATION: U.S. Department of Agriculture, Food Safety and Inspection Service, Washington, DC 20250, USA.

Country: UNITED STATES

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MEDLINETA: J AOAC Int

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