Comparison of microbial receptor assay and liquid chromatography for determination of penicillin G and amoxicillin in milk powder.

Comparison of microbial receptor assay and liquid chromatography for determination of penicillin G and amoxicillin in milk powder. Research Abstract Details 

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Comparison of microbial receptor assay and liquid chromatography for determination of penicillin G and amoxicillin in milk powder. Abstract Text:

A microbial receptor assay (Charm II Tablet Beta-Lactam Test) and liquid chromatography (LC) were compared for determination of penicillin G (PG) and amoxicillin (AMOX) in reconstituted milk powder. Nonfat dry milk and whole dry milk were reconstituted (10%, w/v) to concentrations of 0, 2.5, 5, 7.5, and 10 ppb PG; nonfat dry milk was reconstituted (10%, w/v) to 0, 7.5, 10, and 15 ppb AMOX. Reconstituted samples were analyzed blindly by each method. Concentrations determined by both methods demonstrated good agreement. A significant difference between methods (p < or = 0.05) was observed only for 7.5 ppb PG in defatted dry milk. Significant differences were not observed between known concentrations and concentrations determined by the Charm II assay for PG or AMOX in defatted dry milk and PG in whole dry milk. Results by LC showed significant differences (p < 0.05) between known and measured concentrations at 10 ppb PG in both milks and 0 ppb AMOX in defatted dry milk. These results suggest that both the microbial receptor assay and LC may be useful for determination of PG and AMOX near safe level and tolerance, respectively, in reconstituted milk powder.

Comparison of microbial receptor assay and liquid chromatography for determination of penicillin G and amoxicillin in milk powder. Publishing Authors By Initials

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Comparison of microbial receptor assay and liquid chromatography for determination of penicillin G and amoxicillin in milk powder. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of AOAC International

VOLUME: 85

Page Numbers: 546-50

Journal Abbreviation: J AOAC Int

ISSN: 1060-3271

DAY: 17

MONTH: 03

YEAR: 2008

Comparison of microbial receptor assay and liquid chromatography for determination of penicillin G and amoxicillin in milk powder. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9215446

Comparison of microbial receptor assay and liquid chromatography for determination of penicillin G and amoxicillin in milk powder. Keywords Mesh Terms:

KEYWORDS: Powders

MESH TERMS: analysis

Chemical & Substance for Abstract: Comparison of microbial receptor assay and liquid chromatography for determination of penicillin G and amoxicillin in milk powder. Information

Substance Name: Penicillin G

Registry Number: 61-33-6

Grant and Affiliation Information for Comparison of microbial receptor assay and liquid chromatography for determination of penicillin G and amoxicillin in milk powder.

AFFILIATION: North Carolina State University, College of Veterinary Medicine, Department of Farm Animal Health and Resource Management, Raleigh 27606, USA. kevin_anderson@ncsu.edu

Country: United States

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MEDLINETA: J AOAC Int

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