Comparison of four commercially available rapid test kits with liquid chromatography for detecting penicillin G residues in bovine plasma.

Comparison of four commercially available rapid test kits with liquid chromatography for detecting penicillin G residues in bovine plasma. Research Abstract Details 

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Comparison of four commercially available rapid test kits with liquid chromatography for detecting penicillin G residues in bovine plasma. Abstract Text:

Four commercially available rapid tests (Brilliant Black reduction test, LacTek test, Charm Farm test, and Charm Test II receptor assay) were compared with a liquid chromatographic (LC) method (lowest quantitatable level of 5 ng/mL) in their efficiency, reliability, and sensitivity to detect penicillin G in bovine plasma. Samples were obtained from 16 steers treated with procaine penicillin G alone or in combination with its long-acting form, benzathine penicillin G. The steers were injected intramuscularly with penicillin G doses ranging from label dose to about 9 times label dose. When results of the Brilliant Black reduction, LacTek, Charm Test II, and Charm Farm tests for penicillin G in plasma (with detection sensitivities of 5, 10, 20, and 30 ng/mL, respectively) were compared with results of LC, none of the rapid tests gave false-positive results. Each rapid test elicited a positive response when used to test bovine plasma containing penicillin G residues at concentrations above the test's detection sensitivity. The simplicity, selectivity, and sensitivity of the rapid tests, coupled with rapidity with which results are obtained, make them suitable for use in large-volume preslaughter screening of penicillin-treated cattle.

Comparison of four commercially available rapid test kits with liquid chromatography for detecting penicillin G residues in bovine plasma. Publishing Authors By Initials

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Comparison of four commercially available rapid test kits with liquid chromatography for detecting penicillin G residues in bovine plasma. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of AOAC International

VOLUME: 78

Page Numbers: 1144-52

Journal Abbreviation: J AOAC Int

ISSN: 1060-3271

DAY: 17

MONTH: 03

YEAR: 2008

Comparison of four commercially available rapid test kits with liquid chromatography for detecting penicillin G residues in bovine plasma. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9215446

Comparison of four commercially available rapid test kits with liquid chromatography for detecting penicillin G residues in bovine plasma. Keywords Mesh Terms:

KEYWORDS: Time Factors

MESH TERMS: statistics & numerical data

Chemical & Substance for Abstract: Comparison of four commercially available rapid test kits with liquid chromatography for detecting penicillin G residues in bovine plasma. Information

Substance Name: Penicillin G

Registry Number: 61-33-6

Grant and Affiliation Information for Comparison of four commercially available rapid test kits with liquid chromatography for detecting penicillin G residues in bovine plasma.

AFFILIATION: Agriculture & Agri-Food Canada, Food Production and Inspection Branch, Saskatoon, SK.

Country: UNITED STATES

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MEDLINETA: J AOAC Int

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