COMPARISON OF DIFFERENT PHENOTYPIC METHODS OF DETECTION OF METHICILLIN RESISTANCE IN Staphylococcus aureus WITH THE MOLECULAR DETECTION OF mec-A GENE.

COMPARISON OF DIFFERENT PHENOTYPIC METHODS OF DETECTION OF METHICILLIN RESISTANCE IN Staphylococcus aureus WITH THE MOLECULAR DETECTION OF mec-A GENE. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

COMPARISON OF DIFFERENT PHENOTYPIC METHODS OF DETECTION OF METHICILLIN RESISTANCE IN Staphylococcus aureus WITH THE MOLECULAR DETECTION OF mec-A GENE. Abstract Text:

Mohammad Zeeshan,Kausar Jabeen,Erum Khan,Seema Irfan,Saira Ibrahim,Zeba Parween,Afia Zafar,

Objective: To evaluate accuracy, cost-effectiveness and ease to perform different phenotypic methods i.e. Cefoxitin 30 microg disc, Oxacillin 1microg disc and Oxacillin agar screening plate (6microg/ml ) for early and accurate identification of MRSA by comparing with the detection of mec-A gene in our clinical isolates. Design: A comparative study. Place and Duration of Study: Clinical samples submitted in the Clinical Microbiology Laboratory at Aga Khan University Hospital, Karachi, from 1st August to 31st October 2006. Material and Methods: Out of 200 clinical samples, conventional Polymerase chain reaction (PCR) was done on 62 pure biochemically identified S. aureus isolates for mec-A gene detection. Phenotypic methods for detecting methicillin sensitivity (Cefoxitin 30 microg disc, Oxacillin 1 microg disc and Oxacillin agar screening plate) were also used according to the recommended incubation time, duration and temperature on the same isolates. Results: Out of 62 isolates of S. aureus, mec-A gene were detected (MRSA) in 32, whereas 30 were mec-A gene negative (MSSA). Cefoxitin disc and agar screening plate correctly identify all MRSA isolates with the sensitivity and specificity of 100%. Single isolate was false, positively detected as sensitive with Oxacillin 1microg disc, due to which, the sensitivity and negative predictive value of this method were reduced to 96.9% and 96.8% respectively, while positive predictive value and specificity remained 100%. Conclusion: Comparing different phenotypic methods for MRSA screening in routine microbiology laboratory, Cefoxitin disc and Oxacillin agar screening has better sensitivity and specificity comparative to Oxacillin disc. However, Cefoxitin disc can be preferred especially for small laboratories because it is easy to perform, do not require special technique and media preparation is consequently more cost-effective.

COMPARISON OF DIFFERENT PHENOTYPIC METHODS OF DETECTION OF METHICILLIN RESISTANCE IN Staphylococcus aureus WITH THE MOLECULAR DETECTION OF mec-A GENE. Publishing Authors By Initials

M Zeeshan,K Jabeen,E Khan,S Irfan,S Ibrahim,Z Parween,A Zafar,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

COMPARISON OF DIFFERENT PHENOTYPIC METHODS OF DETECTION OF METHICILLIN RESISTANCE IN Staphylococcus aureus WITH THE MOLECULAR DETECTION OF mec-A GENE. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of the College of Physicians and Surgeons-

VOLUME: 17

Page Numbers: 666-70

Journal Abbreviation:

ISSN: 1022-386X

DAY: 11

MONTH: Nov

YEAR: 2007

COMPARISON OF DIFFERENT PHENOTYPIC METHODS OF DETECTION OF METHICILLIN RESISTANCE IN Staphylococcus aureus WITH THE MOLECULAR DETECTION OF mec-A GENE. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9606447

COMPARISON OF DIFFERENT PHENOTYPIC METHODS OF DETECTION OF METHICILLIN RESISTANCE IN Staphylococcus aureus WITH THE MOLECULAR DETECTION OF mec-A GENE. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: COMPARISON OF DIFFERENT PHENOTYPIC METHODS OF DETECTION OF METHICILLIN RESISTANCE IN Staphylococcus aureus WITH THE MOLECULAR DETECTION OF mec-A GENE. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for COMPARISON OF DIFFERENT PHENOTYPIC METHODS OF DETECTION OF METHICILLIN RESISTANCE IN Staphylococcus aureus WITH THE MOLECULAR DETECTION OF mec-A GENE.

AFFILIATION: Department of Pathology and Microbiology, The Aga Khan University Hospital, Karachi, Pakistan.

Country: Pakistan

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Coll Physicians Surg Pak

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

COMPARISON OF DIFFERENT PHENOTYPIC METHODS OF DETECTION OF METHICILLIN RESISTANCE IN Staphylococcus aureus WITH THE MOLECULAR DETECTION OF mec-A GENE Related Publications

• Anaesthesia for surgical correction of scoliosis with spinal cord monitoring--a case series.
• Bone marrow involvement in Hodgkin's disease: the significance of non-infiltrative changes.
• COMPARISON OF DIFFERENT PHENOTYPIC METHODS OF DETECTION OF METHICILLIN RESISTANCE IN Staphylococcus aureus WITH THE MOLECULAR DETECTION OF mec-A GENE.
• Cephalosporin resistant Shigella flexneri from a clinical isolate--a rare finding.
• Creatine kinase activities in sera of cirrhotics.
• Cytochemistry of leukaemic lymphoid cells.
• Development of agriculture biotechnology in Pakistan.
• Effect of neuraminidase on lymphocyte function in chronic lymphatic leukaemia (CLL).
• Evaluation of immune response to Hepatitis B vaccine in health care workers at a tertiary care hospital in Pakistan: an observational prospective study.
• FAB classification of acute leukaemia.
• FAB classification of acute lymphoblastic leukaemia (ALL) and its relevance to ALL in Karachi children.
• HLA diversity and infections.
• HLA system and applications.
• Hybrid biosorbent: An innovative matrix to enhance the biosorption of Cd(II) from aqueous solution.
• Inhibition of phytohaemagglutinin--induced lymphocyte transformation and mitosis by ethidium bromide.
• Interferons.
• Interferons: in vivo effects.
• Laboratory techniques for examination of intestinal parasites.
• Mental retardation in children.
• Morphology and frequency distribution of protozoan Blastocystis hominis.
• Ocular manifestations of xeroderma pigmentosum.
• Prevalent nosocomial gram negative aerobic bacilli and their antimicrobial susceptibility pattern in intensive care unit.
• Ross syndrome with generalized anhidrosis and localized disabling compensatory hyperhidrosis.
• S.I. units and conversion factors for old units for routine substrates in blood.
• Screening for antibodies to HTLV-III/LAV in our population. A word of caution.
• Seasonal variation in bacterial pathogens isolated from stool samples in Karachi, Pakistan.
• Surgical drains.
• T-lymphoblastic leukaemia (T-ALL).
• Thyroid hormones in liver disease.
• Urologic J.J stents: uses and complications.