Comparison of Albumin Uptake in Rat Alveolar Type II and Type I-like Epithelial Cells in Primary Culture.

Comparison of Albumin Uptake in Rat Alveolar Type II and Type I-like Epithelial Cells in Primary Culture. Research Abstract Details 

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Comparison of Albumin Uptake in Rat Alveolar Type II and Type I-like Epithelial Cells in Primary Culture. Abstract Text:

Mika Ikehata,Ryoko Yumoto,Kosuke Nakamura,Junya Nagai,Mikihisa Takano,

PURPOSE: To elucidate and compare the activity and mechanism of albumin uptake in primary cultured alveolar type II and type I-like epithelial cells. MATERIALS AND METHODS: Type II epithelial cells isolated from rat lungs were cultured for 2 days at 5 x 10(6) cells/35-mm dish or for 6 days at 2 x 10(6) cells/35-mm dish. The mRNA expression of marker genes and FITC-albumin uptake were examined. RESULTS: The cells cultured for 2 days exhibited cuboidal type II epithelial morphology with lamellar bodies inside the cells, while the cells cultured for 6 days exhibited squamous type I epithelial morphology. These morphological characteristics were consistent with the changes in mRNA expression pattern of marker genes. FITC-albumin uptake in both cells was temperature-dependent and was inhibited by metabolic inhibitors and bafilomycin A(1). The rate of uptake was much higher in type II cells than type I-like cells. In both cells, FITC-albumin uptake was inhibited by clathrin mediated-endocytosis inhibitors, but not by caveolae mediated-endocytosis inhibitors. CONCLUSIONS: These findings indicate that albumin in alveolar lining fluid is internalized into type II and type I epithelial cells via clathrin-mediated endocytosis, and the rate of albumin uptake is higher in type II cells than type I cells.

Comparison of Albumin Uptake in Rat Alveolar Type II and Type I-like Epithelial Cells in Primary Culture. Publishing Authors By Initials

M Ikehata,R Yumoto,K Nakamura,J Nagai,M Takano,

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Comparison of Albumin Uptake in Rat Alveolar Type II and Type I-like Epithelial Cells in Primary Culture. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Pharmaceutical research

VOLUME: 25

Page Numbers: 913-22

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ISSN: 0724-8741

DAY: 11

MONTH: 09

YEAR: 2007

Comparison of Albumin Uptake in Rat Alveolar Type II and Type I-like Epithelial Cells in Primary Culture. Information

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LANGUAGE: eng

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AFFILIATION: Department of Pharmaceutics and Therapeutics, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8553, Japan.

Country: United States

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MEDLINETA: Pharm Res

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