Comparison between properties of 2'-O,4'-C-ethylene-bridged nucleic acid (ENA) phosphorothioate oligonucleotides and N3'-P5' thiophosphoramidate oligonucleotides.

Comparison between properties of 2'-O,4'-C-ethylene-bridged nucleic acid (ENA) phosphorothioate oligonucleotides and N3'-P5' thiophosphoramidate oligonucleotides. Research Abstract Details 

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Comparison between properties of 2'-O,4'-C-ethylene-bridged nucleic acid (ENA) phosphorothioate oligonucleotides and N3'-P5' thiophosphoramidate oligonucleotides. Abstract Text:

Makiko Horie,Koji Morita,Junko Kawakami,Osamu Ando,Makoto Koizumi,Shinya Tsutsumi,Makiko Horie,Koji Morita,Junko Kawakami,Osamu Ando,Makoto Koizumi,Shinya Tsutsumi,

Synthesis and properties of an oligonucleotide uniformly modified with 2'-O,4-C-ethylene-bridged nucleic acid (ENA) units were compared with those of GRN163, which is modified with N3'-P5' thiophosphoramidates, with the sequence targeting human telomerase RNA subunit. Although an ENA phosphorothioate oligonucleotide, ENA-13, could be synthesized using ENA phosphoramidites on a 100-mg scale, synthesis of GRN163 was very hard even on a 1-micomol scale. In view of both stability of the duplex formation with complementary RNA and the efficiency of cellular uptake by endocytosis, ENA-13 was superior to GRN163. These findings suggest that ENA-13 has useful properties for antisense therapeutic application.

Comparison between properties of 2'-O,4'-C-ethylene-bridged nucleic acid (ENA) phosphorothioate oligonucleotides and N3'-P5' thiophosphoramidate oligonucleotides. Publishing Authors By Initials

M Horie,K Morita,J Kawakami,O Ando,M Koizumi,S Tsutsumi,M Horie,K Morita,J Kawakami,O Ando,M Koizumi,S Tsutsumi,

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Comparison between properties of 2'-O,4'-C-ethylene-bridged nucleic acid (ENA) phosphorothioate oligonucleotides and N3'-P5' thiophosphoramidate oligonucleotides. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Nucleosides, nucleotides & nucleic acids

VOLUME: 25

Page Numbers: 231-42

Journal Abbreviation: Nucleosides Nucleotides Nuclei

ISSN: 1525-7770

DAY: 24

MONTH: Mar

YEAR: 2006

Comparison between properties of 2'-O,4'-C-ethylene-bridged nucleic acid (ENA) phosphorothioate oligonucleotides and N3'-P5' thiophosphoramidate oligonucleotides. Information

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LANGUAGE: eng

NlmUniqueID: 100892832

Comparison between properties of 2'-O,4'-C-ethylene-bridged nucleic acid (ENA) phosphorothioate oligonucleotides and N3'-P5' thiophosphoramidate oligonucleotides. Keywords Mesh Terms:

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Grant and Affiliation Information for Comparison between properties of 2'-O,4'-C-ethylene-bridged nucleic acid (ENA) phosphorothioate oligonucleotides and N3'-P5' thiophosphoramidate oligonucleotides.

AFFILIATION: Sankyo Co., Ltd., Lead Discovery Research Laboratories, Shinagawa-ku, Tokyo, Japan.

Country: United States

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MEDLINETA: Nucleosides Nucleotides Nuclei

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