Comparison between morphological and staining characteristics of live and dead eggs of Schistosoma mansoni.

Comparison between morphological and staining characteristics of live and dead eggs of Schistosoma mansoni. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Comparison between morphological and staining characteristics of live and dead eggs of Schistosoma mansoni. Abstract Text:

A K Sarvel,J R Kusel,N ,Pmz Coelho,N Katz,A K Sarvel,J R Kusel,N Araújo,Pmz Coelho,N Katz,

Schistosoma mansoni eggs are classified, according to morphological characteristics, as follows: viable mature and immature eggs; dead mature and immature eggs, shells and granulomas. The scope of this study was to compare the staining characteristics of different morphological types of eggs in the presence of fluorescent labels and vital dyes, aiming at differentiating live and dead eggs. The eggs were obtained from the intestines of infected mice, and put into saline 0.85%. The fluorescent labels were Hoechst 33258 and Acridine Orange + Ethidium Bromide and vital dyes (Trypan Blue 0.4% and Neutral Red 1%). When labelled with the probe Hoechst 33258, some immature eggs, morphologically considered viable, presented fluorescence (a staining characteristic detected only in dead eggs); mature eggs did not present fluorescence, and the other types of dead eggs, morphologically defined, showed fluorescence. As far as Acridine Orange + Ethidium Bromide are concerned, either the eggs considered to be live, or the dead ones, presented staining with green color, and only the hatched and motionless miracidium was stained with an orange color. Trypan Blue was not able to stain the eggs, considered to be dead but only dead miracidia which had emerged out of the shell. Neutral Red stained both live and dead eggs. Only the fluorescent Hoechst 33258 can be considered a useful tool for differentiation between dead and live eggs.

Comparison between morphological and staining characteristics of live and dead eggs of Schistosoma mansoni. Publishing Authors By Initials

AK Sarvel,JR Kusel,N ,P Coelho,N Katz,AK Sarvel,JR Kusel,N Araújo,P Coelho,N Katz,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Comparison between morphological and staining characteristics of live and dead eggs of Schistosoma mansoni. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Memórias do Instituto Oswaldo Cruz

VOLUME: 101 Suppl 1

Page Numbers: 289-92

Journal Abbreviation: Mem. Inst. Oswaldo Cruz

ISSN: 0074-0276

DAY: 19

MONTH: Sep

YEAR: 2006

Comparison between morphological and staining characteristics of live and dead eggs of Schistosoma mansoni. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7502619

Comparison between morphological and staining characteristics of live and dead eggs of Schistosoma mansoni. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Comparison between morphological and staining characteristics of live and dead eggs of Schistosoma mansoni. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Comparison between morphological and staining characteristics of live and dead eggs of Schistosoma mansoni.

AFFILIATION: Laboratório de Esquistossomose, Centro de Pesquisas René-Rachou-Fiocruz, 31190-002 Belo Horizonte, MG, Brazil.

Country: Brazil

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Mem Inst Oswaldo Cruz

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Comparison between morphological and staining characteristics of live and dead eggs of Schistosoma mansoni Related Publications

• A distributed computing tool for generating neural simulation databases.
• Attempted suicide among transgender persons: The influence of gender-based discrimination and victimization.
• Biophysical analysis of prototype microbicidal gels.
• Cognitive control and prehospital endotracheal intubation.
• Comparison between morphological and staining characteristics of live and dead eggs of Schistosoma mansoni.
• Correlates of referral practices of general surgeons to plastic surgeons for mastectomy reconstruction.
• Direct observation of computer workplace risk factors of college students.
• Elevation of glucocorticoids is necessary but not sufficient for the escalation of cocaine self-administration by chronic electric footshock stress in rats.
• Genome diversity in microbial eukaryotes.
• Impediment profiling for smoking cessation: application in the worksite.
• Information given to postmenopausal women on coronary computed tomography may influence cardiac risk reduction efforts.
• Interpreting properties of microbicide drug delivery gels: analyzing deployment kinetics due to squeezing.
• Krüppel-like factor 5 activates MEK/ERK signaling via EGFR in primary squamous epithelial cells.
• Massage therapy for osteoarthritis of the knee: a randomized controlled trial.
• Motor innervation of the muscle spindle: the contribution of bernhard katz.
• Neighborhoods matter: use of hospitals with worse outcomes following total knee replacement by patients from vulnerable populations.
• Ocular phenotype in a mouse gene knockout model for infantile neuronal ceroid lipofuscinosis.
• Orthopaedic procedure volume and patient outcomes: a systematic literature review.
• Overexpression of Kruppel-like factor 5 in esophageal epithelia in vivo leads to increased proliferation in basal but not suprabasal cells.
• Plasma cathepsin D isoforms and their active metabolites increase after myocardial infarction and contribute to plasma renin activity.
• Role of membrane potential in calcium signaling during rhythmic bursting in tritonia swim interneurons.
• Short- and long-term response to and weaning from infliximab therapy in pediatric ulcerative colitis.
• Sparse and selective odor coding by mitral/tufted neurons in the main olfactory bulb.
• State-dependent modulation of time-varying gustatory responses.
• Synaptic integration of olfactory information in mouse anterior olfactory nucleus.
• Temporary basolateral amygdala lesions disrupt acquisition of socially transmitted food preferences in rats.
• The influence of race, ethnicity, and individual socioeconomic factors on breast cancer stage at diagnosis.
• The many flavors of temporal coding in gustatory cortex.
• Undergraduate college students' upper extremity symptoms and functional limitations related to computer use: a replication study.
• Understanding HSV-1 entry glycoproteins.