Comparing two microarray platforms for identifying mutated genes in barley (Hordeum vulgare L.).

Comparing two microarray platforms for identifying mutated genes in barley (Hordeum vulgare L.). Research Abstract Details 

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Comparing two microarray platforms for identifying mutated genes in barley (Hordeum vulgare L.). Abstract Text:

Shakhira Zakhrabekova,Simon P Gough,Udda Lundqvist,Mats Hansson,

We have previously described the evaluation of a cDNA microarray platform to identify and clone mutated barley (Hordeum vulgare L.) genes, using their transcriptionally defective mutant alleles (S. Zakhrabekova, C.G. Kannangara, D. von Wettstein, M. Hansson, A microarray approach for identification of mutated genes, Plant Physiol. Biochem. 40 (2002) 189-197). It was concluded that competitive hybridization between phenotypically similar mutants could specifically highlight an arrayed clone, corresponding to the mutated gene. In this study we evaluate whether the Affymetrix microarray platform can be used for the same purpose. The Affymetrix barley microarray contains a large number of clones (22,792 probe sets). In this and the previous study we used two barley mutant strains, xantha-h.57 and xantha-f.27, with known mutations in different subunit genes of the chlorophyll biosynthetic enzyme magnesium chelatase (EC 6.6.1.1). Mutant xantha-h.57 produces no Xantha-h mRNA whereas in xantha-f.27 the nonsense mutation in the last exon of the gene, results in nonsense-mediated decay of Xantha-f mRNA. We conclude that the Affymetrix platform meets our requirements and that our approach successfully highlighted the arrayed Xantha-h clone and that Xantha-f was among the top fourteen candidates.

Comparing two microarray platforms for identifying mutated genes in barley (Hordeum vulgare L.). Publishing Authors By Initials

S Zakhrabekova,SP Gough,U Lundqvist,M Hansson,

For similar nucleic acids, nucleotides, and nucleosides: nucleic acids: rna research abstracts see: nucleic acids, nucleotides, and nucleosides: nucleic acids: rna research

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Comparing two microarray platforms for identifying mutated genes in barley (Hordeum vulgare L.). Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Plant physiology and biochemistry : PPB / Société

VOLUME: 45

Page Numbers: 617-22

Journal Abbreviation: Plant Physiol. Biochem.

ISSN: 0981-9428

DAY: 31

MONTH: 05

YEAR: 2007

Comparing two microarray platforms for identifying mutated genes in barley (Hordeum vulgare L.). Information

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LANGUAGE: eng

NlmUniqueID: 9882449

Comparing two microarray platforms for identifying mutated genes in barley (Hordeum vulgare L.). Keywords Mesh Terms:

KEYWORDS: RNA

MESH TERMS: chemistry

Chemical & Substance for Abstract: Comparing two microarray platforms for identifying mutated genes in barley (Hordeum vulgare L.). Information

Substance Name: magnesium chelatase

Registry Number: EC 4.99.1-

Grant and Affiliation Information for Comparing two microarray platforms for identifying mutated genes in barley (Hordeum vulgare L.).

AFFILIATION: Department of Biochemistry, Lund University, Box 124, SE-22100 Lund, Sweden. shakhira.zakhrabekova@biochemistry.lu.se

Country: France

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MEDLINETA: Plant Physiol Biochem

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