Comparing Insertion Libraries in Two Pseudomonas aeruginosa Strains to Assess Gene Essentiality.

Comparing Insertion Libraries in Two Pseudomonas aeruginosa Strains to Assess Gene Essentiality. Research Abstract Details 

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Comparing Insertion Libraries in Two Pseudomonas aeruginosa Strains to Assess Gene Essentiality. Abstract Text:

Nicole T Liberati,Jonathan M Urbach,Tara K Thurber,Gang Wu,Frederick M Ausubel,

Putative essential genes can be identified by comparing orthologs not disrupted in multiple near-saturated transposon insertion mutation libraries in related strains of the same bacterial species. Methods for identifying all orthologs between two bacterial strains and putative essential orthologs are described. In addition, protocols detailing near-saturation transposon insertion mutagenesis of bacteria are presented, including (1) conjugation-mediated mutagenesis, (2) automated colony picking and liquid handling of mutant cultures, and (3) arbitrary polymerase chain reaction amplification and sequencing of genomic DNA adjacent to transposon insertion sites.

Comparing Insertion Libraries in Two Pseudomonas aeruginosa Strains to Assess Gene Essentiality. Publishing Authors By Initials

NT Liberati,JM Urbach,TK Thurber,G Wu,FM Ausubel,

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Comparing Insertion Libraries in Two Pseudomonas aeruginosa Strains to Assess Gene Essentiality. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Methods in molecular biology (Clifton, N.J.)

VOLUME: 416

Page Numbers: 153-70

Journal Abbreviation: Methods Mol. Biol.

ISSN: 1064-3745

DAY: 21

MONTH: 02

YEAR: 2007

Comparing Insertion Libraries in Two Pseudomonas aeruginosa Strains to Assess Gene Essentiality. Information

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LANGUAGE: eng

NlmUniqueID: 9214969

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MEDLINETA: Methods Mol Biol

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