Comparative transport efficiencies of urea analogues through urea transporter UT-B.

Comparative transport efficiencies of urea analogues through urea transporter UT-B. Research Abstract Details 

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Comparative transport efficiencies of urea analogues through urea transporter UT-B. Abstract Text:

Dan Zhao,N D Sonawane,Marc H Levin,Baoxue Yang,

Expression of urea transporter UT-B confers high urea permeability to mammalian erythrocytes. Erythrocyte membranes also permeate various urea analogues, suggesting common transport pathways for urea and structurally similar solutes. In this study, we examined UT-B-facilitated passage of urea analogues and other neutral small solutes by comparing transport properties of wildtype to UT-B-deficient mouse erythrocytes. Stopped-flow light-scattering measurements indicated high UT-B permeability to urea and chemical analogues formamide, acetamide, methylurea, methylformamide, ammonium carbamate, and acrylamide, each with P(s)>5.0 x 10(-6) cm/s at 10 degrees C. UT-B genetic knockout and phloretin treatment of wildtype erythrocytes similarly reduced urea analogue permeabilities. Strong temperature dependencies of formamide, acetamide, acrylamide and butyramide transport across UT-B-null membranes (E(a)>10 kcal/mol) suggested efficient diffusion of these amides across lipid bilayers. Urea analogues dimethylurea, acryalmide, methylurea, thiourea and methylformamide inhibited UT-B-mediated urea transport by >60% in the absence of transmembrane analogue gradients, supporting a pore-blocking mechanism of UT-B inhibition. Differential transport efficiencies of urea and its analogues through UT-B provide insight into chemical interactions between neutral solutes and the UT-B pore.

Comparative transport efficiencies of urea analogues through urea transporter UT-B. Publishing Authors By Initials

D Zhao,ND Sonawane,MH Levin,B Yang,

For similar organic chemicals: urea research abstracts see: organic chemicals: urea research

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Comparative transport efficiencies of urea analogues through urea transporter UT-B. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biochimica et biophysica acta

VOLUME: 1768

Page Numbers: 1815-21

Journal Abbreviation: Biochim. Biophys. Acta

ISSN: 0006-3002

DAY: 18

MONTH: 04

YEAR: 2007

Comparative transport efficiencies of urea analogues through urea transporter UT-B. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 217513

Comparative transport efficiencies of urea analogues through urea transporter UT-B. Keywords Mesh Terms:

KEYWORDS: Urea

MESH TERMS: metabolism

Chemical & Substance for Abstract: Comparative transport efficiencies of urea analogues through urea transporter UT-B. Information

Substance Name: Acrylamide

Registry Number: 79-06-1

Grant and Affiliation Information for Comparative transport efficiencies of urea analogues through urea transporter UT-B.

AFFILIATION: Department of Medicine, 1246 Health Sciences East Tower, University of California, San Francisco, CA 94143-0521, USA.

Country: Netherlands

AGENCY: United States NIDDK

GRANT: DK-66194

ACRONYM: DK

MEDLINETA: Biochim Biophys Acta

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