Comparative three-dimensional imaging of living neurons with confocal and atomic force microscopy.

Comparative three-dimensional imaging of living neurons with confocal and atomic force microscopy. Research Abstract Details 

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Comparative three-dimensional imaging of living neurons with confocal and atomic force microscopy. Abstract Text:

Helen A McNally,Bartek Rajwa,Jennie Sturgis,J Paul Robinson,

Atomic force microscopy applications extend across a number of fields; however, limitations have reduced its effectiveness in live cell analysis. This report discusses the use of AFM to evaluate the three-dimensional (3-D) architecture of living chick dorsal root ganglia and sympathetic ganglia. These data sets were compared to similar images acquired with confocal laser scanning microscopy of identical cells. For this comparison we made use of visualization techniques which were applicable to both sets of data and identified several issues when coupling these technologies. These direct comparisons offer quantitative validation and confirmation of the character of novel images acquired by AFM. This paper is one in a series emphasizing various new applications of AFM in neurobiology.

Comparative three-dimensional imaging of living neurons with confocal and atomic force microscopy. Publishing Authors By Initials

HA McNally,B Rajwa,J Sturgis,JP Robinson,

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Comparative three-dimensional imaging of living neurons with confocal and atomic force microscopy. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of neuroscience methods

VOLUME: 142

Page Numbers: 177-84

Journal Abbreviation: J. Neurosci. Methods

ISSN: 0165-0270

DAY: 30

MONTH: Mar

YEAR: 2005

Comparative three-dimensional imaging of living neurons with confocal and atomic force microscopy. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Comparative three-dimensional imaging of living neurons with confocal and atomic force microscopy.

AFFILIATION: Center for Paralysis Research, School of Veterinary Medicine, Purdue University, West Lafayette, IN 47906-2065, USA.

Country: Netherlands

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MEDLINETA: J Neurosci Methods

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