Amino acid sequence analysis was done on a human lambda Bence Jones protein NIG-64 with the major objective of determining the sequence of the variable region. Nineteen tryptic peptides covering 216 residues were isolated from the completely reduced and aminoethylated protein, and 17 of these were completely sequenced. These comprised the entire variable region and 11 from the constant region. For the remaining peptides covering the rest of the constant region, only partial sequences or the amino acid compositions were determined. All the tryptic peptides could be arranged in order on the basis of the above results and homology with other human lambda light chains of the same isotype. The sequence of the variable region of the protein is highly homologous with that of protein New of subgroup V lambda I as compared with other proteins of the same subgroup, suggesting that subgroup V lambda I may be further divided into subsubgroups, namely subsubgroups V lambda I-1 and V lambda I-2.
Comparative studies on the structure of the light chains of human immunoglobulins. IV. Assignment of a subsubgroup. Publishing Authors By Initials
Comparative studies on the structure of the light chains of human immunoglobulins. IV. Assignment of a subsubgroup. Journal Published:
PUBLICATION TYPE: Research Support, Non-U.S. Gov
Journal: Journal of biochemistry
VOLUME: 93
Page Numbers: 421-9
Journal Abbreviation: J. Biochem.
ISSN: 0021-924X
DAY: 19
MONTH: Feb
YEAR: 1983
Comparative studies on the structure of the light chains of human immunoglobulins. IV. Assignment of a subsubgroup. Information
Number of References:
LANGUAGE: eng
NlmUniqueID: 376600
Comparative studies on the structure of the light chains of human immunoglobulins. IV. Assignment of a subsubgroup. Keywords Mesh Terms:
KEYWORDS: Trypsin
MESH TERMS: immunology
Chemical & Substance for Abstract: Comparative studies on the structure of the light chains of human immunoglobulins. IV. Assignment of a subsubgroup. Information
Substance Name: Trypsin
Registry Number: EC 3.4.21.4
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Country: JAPAN
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MEDLINETA: J Biochem
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