Comparative proteomics of cell division mutants and wild-type of Synechococcus sp. strain PCC 7942.

Comparative proteomics of cell division mutants and wild-type of Synechococcus sp. strain PCC 7942. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Comparative proteomics of cell division mutants and wild-type of Synechococcus sp. strain PCC 7942. Abstract Text:

Olga A Koksharova,Johan Klint,Ulla Rasmussen,

Bacterial cell division is a highly co-ordinated and fine-tuned process. In the unicellular cyanobacterium Synechococcus sp. strain PCC 7942, inactivating mutations in the ftn2 and ftn6 genes block cell division and result in a phenotype with extensively elongated cells. In order to establish the pleiotropic responses induced and cellular processes affected by blocked cell division, the proteomes of wild-type and the cell division mutants Ftn2 and Ftn6 of Synechococcus sp. strain PCC 7942 were characterized and compared. By separating soluble extracted proteins on 2D gels, more than 800 protein spots were visualized on each SYPRO Ruby-stained gel. Quantitative differences in protein composition were detected by using the PDQuest software, and comparative analysis revealed that 76 protein spots changed significantly in the cell division mutants. These protein spots were selected for identification using peptide mass fingerprints generated by MALDI-TOF MS. Fifty-three protein spots were successfully identified, representing 44 different proteins. The upregulated proteins include proteins involved in cell division/cell morphogenesis, protein synthesis and processing, oxidative stress response, amino acid metabolism, nucleotide biosynthesis, and glycolysis, as well as unknown proteins. Among the downregulated proteins are those involved in chromosome segregation, protein processing, photosynthesis, redox regulation, carbon dioxide fixation, nucleotide biosynthesis, the biosynthetic pathway to fatty acids, and energy production. Besides eliciting common responses, inactivation of Ftn2 and Ftn6 in the mutants may result in different responses in protein levels between the mutants. Among 18 identified differentially affected protein spots, 75 % (9/12) of the protein spots affected in the Ftn2 mutant were upshifted, whereas in the Ftn6 mutant 70 % (7/10) of the affected protein spots were downshifted. Identification of such differentially expressed proteins provides new targets for future studies that will allow assessment of their physiological roles and significance in cyanobacterial cell division.

Comparative proteomics of cell division mutants and wild-type of Synechococcus sp. strain PCC 7942. Publishing Authors By Initials

OA Koksharova,J Klint,U Rasmussen,

For similar bacteria: cyanobacteria: synechococcus research abstracts see: bacteria: cyanobacteria: synechococcus research

PUBMED ID PMID:

MEDLINE DATE:

Comparative proteomics of cell division mutants and wild-type of Synechococcus sp. strain PCC 7942. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Microbiology (Reading, England)

VOLUME: 153

Page Numbers: 2505-17

Journal Abbreviation: Microbiology (Reading, Engl.)

ISSN: 1350-0872

DAY: 27

MONTH: Aug

YEAR: 2007

Comparative proteomics of cell division mutants and wild-type of Synechococcus sp. strain PCC 7942. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9430468

Comparative proteomics of cell division mutants and wild-type of Synechococcus sp. strain PCC 7942. Keywords Mesh Terms:

KEYWORDS: Synechococcus

MESH TERMS: genetics

Chemical & Substance for Abstract: Comparative proteomics of cell division mutants and wild-type of Synechococcus sp. strain PCC 7942. Information

Substance Name: Proteome

Registry Number: 0

Grant and Affiliation Information for Comparative proteomics of cell division mutants and wild-type of Synechococcus sp. strain PCC 7942.

AFFILIATION: Department of Botany, Stockholm University, SE-106 91 Stockholm, Sweden. OA-Koksharova@rambler.ru

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Microbiology

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Comparative proteomics of cell division mutants and wild-type of Synechococcus sp strain PCC 7942 Related Publications

• A Chronic Increase in Energy Expenditure Inhibits S6K1 and Reduces IRS-1 Serine Phosphorylation in Muscle: 540: May 30 10:30 AM - 10:45 AM.
• Association between intelligence and coronary heart disease mortality: a population-based cohort study of 682 361 Swedish men.
• Association of maternal obesity before conception with poor lactation performance.
• Blood Flow Restriction during Low-Intensity Resistance Exercise Increases Muscle Protein Synthesis through the mTOR Signaling Pathway: 666: May 31 8:00 AM 8:15 AM.
• Characterization of carbohydrate structures of bovine MUC15 and distribution of the mucin in bovine milk.
• Comparative proteomics of cell division mutants and wild-type of Synechococcus sp. strain PCC 7942.
• Cranial implant design using augmented reality immersive system.
• Distinct distribution of autosomal dominant spinocerebellar ataxia in the Mexican population.
• Dysregulation of muscle fatty acid metabolism in type 2 diabetes is independent of malonyl-CoA.
• Experiences with carrier-mediated transport in liquid-phase microextraction.
• Extraction across supported liquid membranes by use of electrical fields.
• Genome-wide reprogramming in hybrids of somatic cells and embryonic stem cells.
• Haptic rendering of volumetric data for cranial implant modeling.
• Hysterectomy is associated with stress incontinence in women who previously had a transcervical endometrial resection.
• Impulse control disorders in adults with obsessive compulsive disorder.
• Inhibitory activities of bovine macromolecular whey proteins on rotavirus infections in vitro and in vivo.
• Inhibitory effects of human and bovine milk constituents on rotavirus infections.
• Late-onset obsessive compulsive disorder: clinical characteristics and psychiatric comorbidity.
• Mechanisms controlling death, survival and proliferation in a model unicellular eukaryote Tetrahymena thermophila.
• Microemulsion electrokinetic chromatography in suppressed electroosmotic flow environment. Separation of fat-soluble vitamins.
• Muscle Protein Synthesis is Enhanced Post-Resistance Exercise by Nutrient Stimulation of the mTOR Signaling Pathway: 873: June 1 1:30 PM - 1:45 PM.
• Olanzapine-induced weight gain and increased visceral adiposity is blocked by melatonin replacement therapy in rats.
• Opening rates of DNA hairpins: experiment and model.
• Postoperative cognitive dysfunction in patients with preoperative cognitive impairment: which domains are most vulnerable?
• Sexual obsessions and clinical correlates in adults with obsessive-compulsive disorder.
• Simulation of eye disease in virtual reality.
• Small RNAs controlling outer membrane porins.
• Stress distributions in diblock copolymers.
• Taboo thoughts and doubt/checking: a refinement of the factor structure for obsessive-compulsive disorder symptoms.
• The brown longitudinal obsessive compulsive study: treatments received and patient impressions of improvement.