Comparative proteomic and transcriptomic profiling of the fission yeast Schizosaccharomyces pombe.

Comparative proteomic and transcriptomic profiling of the fission yeast Schizosaccharomyces pombe. Research Abstract Details 

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Comparative proteomic and transcriptomic profiling of the fission yeast Schizosaccharomyces pombe. Abstract Text:

Michael W Schmidt,Andres Houseman,Alexander R Ivanov,Dieter A Wolf,

The fission yeast Schizosaccharomyces pombe is a widely used model organism to study basic mechanisms of eukaryotic biology, but unlike other model organisms, its proteome remains largely uncharacterized. Using a shotgun proteomics approach based on multidimensional prefractionation and tandem mass spectrometry, we have detected approximately 30% of the theoretical fission yeast proteome. Applying statistical modelling to normalize spectral counts to the number of predicted tryptic peptides, we have performed label-free quantification of 1465 proteins. The fission yeast protein data showed considerable correlations with mRNA levels and with the abundance of orthologous proteins in budding yeast. Functional pathway analysis indicated that the mRNA-protein correlation is strong for proteins involved in signalling and metabolic processes, but increasingly discordant for components of protein complexes, which clustered in groups with similar mRNA-protein ratios. Self-organizing map clustering of large-scale protein and mRNA data from fission and budding yeast revealed coordinate but not always concordant expression of components of functional pathways and protein complexes. This finding reaffirms at the protein level the considerable divergence in gene expression patterns of the two model organisms that was noticed in previous transcriptomic studies.

Comparative proteomic and transcriptomic profiling of the fission yeast Schizosaccharomyces pombe. Publishing Authors By Initials

MW Schmidt,A Houseman,AR Ivanov,DA Wolf,

For similar investigative techniques: chemistry, analytical: mass spectrometry: tandem mass spectrometry research abstracts see: investigative techniques: chemistry, analytical: mass spectrometry: tandem mass spectrometry research

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Comparative proteomic and transcriptomic profiling of the fission yeast Schizosaccharomyces pombe. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Molecular systems biology

VOLUME: 3

Page Numbers: 79

Journal Abbreviation: Mol. Syst. Biol.

ISSN: 1744-4292

DAY: 13

MONTH: 02

YEAR: 2007

Comparative proteomic and transcriptomic profiling of the fission yeast Schizosaccharomyces pombe. Information

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LANGUAGE: eng

NlmUniqueID: 101235389

Comparative proteomic and transcriptomic profiling of the fission yeast Schizosaccharomyces pombe. Keywords Mesh Terms:

KEYWORDS: Tandem Mass Spectrometry

MESH TERMS: genetics

Chemical & Substance for Abstract: Comparative proteomic and transcriptomic profiling of the fission yeast Schizosaccharomyces pombe. Information

Substance Name: Schizosaccharomyces pombe Proteins

Registry Number: 0

Grant and Affiliation Information for Comparative proteomic and transcriptomic profiling of the fission yeast Schizosaccharomyces pombe.

AFFILIATION: NIEHS Center for Environmental Health Proteomics Facility, Harvard School of Public Health, Boston, MA 02115, USA.

Country: England

AGENCY: United States NIGMS

GRANT: GM59780

ACRONYM: GM

MEDLINETA: Mol Syst Biol

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