Comparative proteomic analysis of human osteosarcoma and SV40-immortalized normal osteoblastic cell lines.

Comparative proteomic analysis of human osteosarcoma and SV40-immortalized normal osteoblastic cell lines. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Comparative proteomic analysis of human osteosarcoma and SV40-immortalized normal osteoblastic cell lines. Abstract Text:

Qian-Chen Guo,Jing-Nan Shen,Song Jin,Jin Wang,Gang Huang,Long-Juan Zhang,Gang Huang,Jun-Qiang Yin,Chang-Ye Zou,Ming-Tao Li,

AIM: Comparative proteomics provide a powerful approach in screening for alterations in protein levels and post-translational modifications that are associated with tumors. In the present study, we aimed to identify candidate biomarkers to distinguish osteosarcoma (OS) cells from normal osteoblastic cells. METHODS: We employed 3 OS cell lines (U2OS, IOR/OS9, and SaOS-2), and used the SV40-immortalized normal osteoblastic cell line (hFOB1.19) as the control. The differential protein levels in OS and osteoblastic cells were identified using 2-D gel electrophoresis followed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry analyses. Two proteins of interest, the levels of which were significantly increased in OS cells, were further characterized by Western blot analyses. RESULTS: Twenty-six proteins were identified, the expression level of which was either significantly increased or decreased in the OS cells as compared to the control cells. The expression level of the activator of 90 kDa shock protein ATPase homolog 1 (AHA1), was enhanced 12.4-, 24.1-, and 23.8-fold in SaOS-2, IOR/OS9, and U2OS cells, respectively, and the level of the stomatin-like protein 2 (SLP-2) was increased by 10.4- and 7.8-fold in IOR/OS9 and U2OS cells, respectively, as compared to normal osteoblastic cells. Those observations were confirmed by Western blot analyses. CONCLUSION: A differential proteomic analysis was successfully used to identify AHA1 and SLP-2 that were significantly overproduced in OS cells as compared to normal osteoblastic cells, suggesting that those proteins among others may be effective biomarker candidates for the identification of OS cells.

Comparative proteomic analysis of human osteosarcoma and SV40-immortalized normal osteoblastic cell lines. Publishing Authors By Initials

QC Guo,JN Shen,S Jin,J Wang,G Huang,LJ Zhang,G Huang,JQ Yin,CY Zou,MT Li,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Comparative proteomic analysis of human osteosarcoma and SV40-immortalized normal osteoblastic cell lines. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Acta pharmacologica Sinica

VOLUME: 28

Page Numbers: 850-8

Journal Abbreviation: Acta Pharmacol. Sin.

ISSN: 1671-4083

DAY: 17

MONTH: Jun

YEAR: 2007

Comparative proteomic analysis of human osteosarcoma and SV40-immortalized normal osteoblastic cell lines. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100956087

Comparative proteomic analysis of human osteosarcoma and SV40-immortalized normal osteoblastic cell lines. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Comparative proteomic analysis of human osteosarcoma and SV40-immortalized normal osteoblastic cell lines. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Comparative proteomic analysis of human osteosarcoma and SV40-immortalized normal osteoblastic cell lines.

AFFILIATION: Department of Orthopedic Surgery, Sun Yat-sen University, Guangzhou 510080, China.

Country: China

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Acta Pharmacol Sin

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Comparative proteomic analysis of human osteosarcoma and SV40-immortalized normal osteoblastic cell lines Related Publications

• Airway epithelium and mucus: intracellular signaling pathways for gene expression and secretion.
• Imaging diagnosis of enlarged superior ophthalmic vein.
• Lung autotransplantation technique for treating central lung cancer of upper lobe.
• Study on the relationship between opioid receptors and fetal distress.
• The clinical value of rearrangement of IgH gene and bcl-2/J(H) fuse gene in the diagnosis of orbital lymphoproliferative disorders.
• The effect of metal copper on fracture healing in chicken.
• Transforming growth factor-beta -Smad signaling pathway cooperates with NF-kappa B to mediate nontypeable Haemophilus influenzae-induced MUC2 mucin transcription.
• [Advances in research on ion-channel gating mechanism]
• [Association of dendritic cell infiltration with prognosis and lymph node metastasis of renal cell carcinoma]
• [Changes in serum concentration of podophyllotoxin after its topical application with liposome on rat skin]
• [ECG signal compression based on best basis of wavelet packets]
• [Effect of lovastatin on proliferation and extracellular matrix secretion of rat hepatic stellate cells in vitro]
• [Effects of hypoxic exposure on coordinative expression of cytochrome oxidase subunits I and IV in rat cerebral cortex]
• [Effects of nitric oxide on pleural lymphatic stomata and lymphatic drainage in the rat]
• [Evaluation of p53, p21waf1, and PCNA in the diagnosis on lung cancer]
• [Evaluation the pathological diagnostic values of koilocyte in condyloma acuminatum]
• [Gap junctions in the nervous tissue]
• [Genotyping of HIV resistant alleles in indigenous Tibetan ethnic group of China: low frequency of CCR5triangle32 and high frequency of CCR2b-64I alleles]
• [Logistic regression analysis of wedge-shaped defects in the middle-aged and elderly]
• [Molecular genetic basis of Gilbert's syndrome]
• [Observation on the effect of disinfection to HBsAg by electrolyzed oxidizing water]
• [P2X purinoceptors and their regulations]
• [Preparation of rhBMP-2/BCB reconstituted bone xenograft and assay of its osteoinductivity]
• [Selective transmission of hepatitis B virus variant with S gene mutation between HBV-carrier mothers and their infants]
• [Surgical repair of postinfarction ventricular septal rupture]
• [Surgical treatment of anomalous origin of the left coronary artery from the pulmonary artery]
• [The correlations between plasma TNF-alpha, NO, NOS levels and brain lateralization in mice]
• [The genotype analysis of glucose-6-phosphate dehydrogenase deficiency in Yunnan province]
• [The relationship of low density lipoprotein receptor gene polymorphism and hyperlipidemia]
• [Umbilical cord blood transplantation (UCBT) in thalassemia children]