Comparative genomic hybridization and physiological characterization of environmental isolates indicate that significant (eco-)physiological properties are highly conserved in the species Escherichia coli.

Comparative genomic hybridization and physiological characterization of environmental isolates indicate that significant (eco-)physiological properties are highly conserved in the species Escherichia coli. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Comparative genomic hybridization and physiological characterization of environmental isolates indicate that significant (eco-)physiological properties are highly conserved in the species Escherichia coli. Abstract Text:

Julian Ihssen,Elena Grasselli,Claudio Bassin,Patrice ,Jean-Claude Piffaretti,Wolfgang ,Jacques Schrenzel,Thomas Egli,

Escherichia coli, the common inhabitant of the mammalian intestine, exhibits considerable intraspecies genomic variation, which has been suggested to reflect adaptation to different ecological niches. Also, regulatory trade-offs, e.g. between catabolic versatility and stress protection, are thought to result in significant physiological differences between strains. For these reasons, the relevance of experimental observations made for 'domesticated' E. coli strains with regard to the behaviour of this species in its natural environments is often questioned and doubts are frequently raised on the status of E. coli as a defined species. The variability of important (eco-)physiological functions, such as carbon substrate uptake and breakdown capabilities, as well as stress defence mechanisms, in the genomes of commensal and pathogenic E. coli strains were therefore investigated. Furthermore, (eco-)physiological properties of environmental strains were compared to standard laboratory strain K-12 MG1655. Catabolic, stress protection, and carbon- and energy source transport operons showed a very low intraspecies variability in 57 commensal and pathogenic E. coli. Environmental isolates adapted to glucose-limited growth in a similar way as E. coli MG1655, namely by increasing their catabolic flexibility and by inducing high-affinity substrate uptake systems. The results obtained indicate that significant (eco-)physiological properties are highly conserved in the natural population of E. coli. This questions the proposed dominant role of horizontal gene transfer for niche adaptation.

Comparative genomic hybridization and physiological characterization of environmental isolates indicate that significant (eco-)physiological properties are highly conserved in the species Escherichia coli. Publishing Authors By Initials

J Ihssen,E Grasselli,C Bassin,P ,JC Piffaretti,W ,J Schrenzel,T Egli,

For similar information science: information services: documentation: molecular sequence data research abstracts see: information science: information services: documentation: molecular sequence data research

PUBMED ID PMID:

MEDLINE DATE:

Comparative genomic hybridization and physiological characterization of environmental isolates indicate that significant (eco-)physiological properties are highly conserved in the species Escherichia coli. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Microbiology (Reading, England)

VOLUME: 153

Page Numbers: 2052-66

Journal Abbreviation: Microbiology (Reading, Engl.)

ISSN: 1350-0872

DAY: 27

MONTH: Jul

YEAR: 2007

Comparative genomic hybridization and physiological characterization of environmental isolates indicate that significant (eco-)physiological properties are highly conserved in the species Escherichia coli. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9430468

Comparative genomic hybridization and physiological characterization of environmental isolates indicate that significant (eco-)physiological properties are highly conserved in the species Escherichia coli. Keywords Mesh Terms:

KEYWORDS: Molecular Sequence Data

MESH TERMS: metabolism

Chemical & Substance for Abstract: Comparative genomic hybridization and physiological characterization of environmental isolates indicate that significant (eco-)physiological properties are highly conserved in the species Escherichia coli. Information

Substance Name: Glucose

Registry Number: 50-99-7

Grant and Affiliation Information for Comparative genomic hybridization and physiological characterization of environmental isolates indicate that significant (eco-)physiological properties are highly conserved in the species Escherichia coli.

AFFILIATION: Swiss Federal Institute for Environmental Science and Technology, PO Box 611, Uberlandstrasse 133, CH-8600 Dübendorf, Switzerland.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Microbiology

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Comparative genomic hybridization and physiological characterization of environmental isolates indicate that significant eco-physiological properties are highly conserved in the species Escherichia coli Related Publications

• A left-handed supramolecular assembly around a right-handed screw axis in the crystal structure of homo-DNA.
• Backbone-base inclination as a fundamental determinant of nucleic acid self- and cross-pairing.
• Belowground ectomycorrhizal communities in three Norway spruce stands with different degrees of decline in the Czech Republic.
• Comparative genomic hybridization and physiological characterization of environmental isolates indicate that significant (eco-)physiological properties are highly conserved in the species Escherichia coli.
• Crystal research.
• Crystal structure of tricyclo-DNA: an unusual compensatory change of two adjacent backbone torsion angles.
• Crystal structure, stability and in vitro RNAi activity of oligoribonucleotides containing the ribo-difluorotoluyl nucleotide: insights into substrate requirements by the human RISC Ago2 enzyme.
• Flow-cytometric total bacterial cell counts as a descriptive microbiological parameter for drinking water treatment processes.
• Growth of Vibrio cholerae O1 Ogawa Eltor in freshwater.
• Physician awareness of male osteopenia.
• Quantification of the filterability of freshwater bacteria through 0.45, 0.22, and 0.1 microm pore size filters and shape-dependent enrichment of filterable bacterial communities.
• Selenium modification of nucleic acids: preparation of oligonucleotides with incorporated 2'-SeMe-uridine for crystallographic phasing of nucleic acid structures.
• Selenium modification of nucleic acids: preparation of phosphoroselenoate derivatives for crystallographic phasing of nucleic acid structures.
• Structural rationalization of a large difference in RNA affinity despite a small difference in chemistry between two 2'-O-modified nucleic acid analogues.
• Structure and Activity of Y-class DNA Polymerase DPO4 from Sulfolobus solfataricus with Templates Containing the Hydrophobic Thymine Analog 2,4-Difluorotoluene.
• Water-nucleobase "stacking": H-pi and lone pair-pi interactions in the atomic resolution crystal structure of an RNA pseudoknot.
• [Benign prostatic hyperplasia: anamnesis and indications for surgery]
• [Bladder tumors]
• [Current developments in postoperative pain management]
• [Diabetes]
• [Histochemical studies of amylophosphorylase in the mouse liver. Physiological daily variations and postnatal development]
• [Nephrotic syndrome in childhood]
• [Not Available.]
• [Nurse's role in the oral hygiene of her patients]
• [Psychomedical aspects of back pain (author's transl)]
• [Reflux nephropathy: vesico-ureteral reflux and renal scars]
• [Report on the experience with long-term anticoagulant treatment]
• [Short stories. As long as there is life...]
• [Treatment of vomiting in infants and children with itinerol B6]
• [When and why are antibiotics indicated in airway infections (except pneumonia)?]