Comparative Genetics of Sex Determination: Masculinizing Mutations in Caenorhabditis briggsae.

Comparative Genetics of Sex Determination: Masculinizing Mutations in Caenorhabditis briggsae. Research Abstract Details 

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Comparative Genetics of Sex Determination: Masculinizing Mutations in Caenorhabditis briggsae. Abstract Text:

Danielle F Kelleher,Carlos Egydio de Carvalho,Alana V Doty,Marnie Layton,Andy T Cheng,Laura D Mathies,Dave Pilgrim,Eric S Haag,

The nematodes Caenorhabditis elegans and C. briggsae independently evolved self-fertile hermaphroditism from gonochoristic ancestors. C. briggsae has variably divergent orthologs of nearly all genes in the C. elegans sex determination pathway. Their functional characterization has generally relied on reverse genetic approaches, such as RNA interference and cross-species transgene rescue and more recently on deletion mutations. We have taken an unbiased forward mutagenesis approach to isolating zygotic mutations that masculinize all tissues of C. briggsae hermaphrodites. The screens identified loss-of-function mutations in the C. briggsae orthologs of tra-1, tra-2, and tra-3. The somatic and germline phenotypes of these mutations are largely identical to those of their C. elegans homologs, including the poorly understood germline feminization of tra-1(lf) males. This overall conservation of Cb-tra phenotypes is in contrast to the fem genes, with which they directly interact and which are significantly divergent in germline function. In addition, we show that in both C. briggsae and C. elegans large C-terminal truncations of TRA-1 that retain the DNA-binding domain affect sex determination more strongly than somatic gonad development. Beyond these immediate results, this collection of mutations provides an essential foundation for further comparative genetic analysis of the Caenorhabditis sex determination pathway.

Comparative Genetics of Sex Determination: Masculinizing Mutations in Caenorhabditis briggsae. Publishing Authors By Initials

DF Kelleher,CE de Carvalho,AV Doty,M Layton,AT Cheng,LD Mathies,D Pilgrim,ES Haag,

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Comparative Genetics of Sex Determination: Masculinizing Mutations in Caenorhabditis briggsae. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Genetics

VOLUME: 178

Page Numbers: 1415-29

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ISSN: 0016-6731

DAY: 1

MONTH: 02

YEAR: 2008

Comparative Genetics of Sex Determination: Masculinizing Mutations in Caenorhabditis briggsae. Information

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LANGUAGE: eng

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AFFILIATION: Department of Genetics, North Carolina State University, Raleigh, North Carolina 27695.

Country: United States

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MEDLINETA: Genetics

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