Comparative analysis of genes regulated in acute myelomonocytic leukemia with and without inv(16)(p13q22) using microarray techniques, real-time PCR, immunohistochemistry, and flow cytometry immunophenotyping.

Comparative analysis of genes regulated in acute myelomonocytic leukemia with and without inv(16)(p13q22) using microarray techniques, real-time PCR, immunohistochemistry, and flow cytometry immunophenotyping. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Comparative analysis of genes regulated in acute myelomonocytic leukemia with and without inv(16)(p13q22) using microarray techniques, real-time PCR, immunohistochemistry, and flow cytometry immunophenotyping. Abstract Text:

Xiaoping Sun,Wei Zhang,Latha Ramdas,David N Stivers,Daniel M Jones,Hagop M Kantarjian,Elihu H Estey,Saroj Vadhan-Raj,L Jeffrey Medeiros,Carlos E Bueso-Ramos,

Acute myeloid leukemia with inv(16)(p13q22), also known as M4Eo, is a distinct type of leukemia with characteristic clinicopathologic and cytogenetic features. Patients with M4Eo have monocytosis, high blast counts, and abnormal bone marrow eosinophils that contain large basophilic granules. The inv(16)(p13q22) or, less commonly, the t(16;16)(p13;q22) causes fusion of the CBFbeta gene at 16q22 and the MYH11 gene at 16p13, creating the novel chimeric protein CBFbeta-MYH11. To understand the underlying molecular mechanisms unique to M4Eo biology, we determined the gene expression profile of M4Eo cases by using cDNA and long oligonucleotide microarrays. Cases of acute myelomonocytic leukemia without CBFbeta-MYH11 (M4) acted as our control. We found that in the gene expression profile of M4Eo, NF-kappaB activators and inhibitors were upregulated and downregulated, respectively, suggesting that the NF-kappaB signaling pathway is activated at a higher level in M4Eo than in acute myelomonocytic leukemia M4. In addition, the gene expression profile of M4Eo indicates high cell proliferation and low apoptosis. We used real-time PCR, immunohistochemistry, and flow cytometry immunophenotyping to confirm some of our microarray data. These findings most likely represent the functional consequences of the abnormal chimeric protein CBFbeta-MYH11, which is unique to this disease, and suggest that NF-kappaB is a potential therapeutic target for treating M4Eo patients.

Comparative analysis of genes regulated in acute myelomonocytic leukemia with and without inv(16)(p13q22) using microarray techniques, real-time PCR, immunohistochemistry, and flow cytometry immunophenotyping. Publishing Authors By Initials

X Sun,W Zhang,L Ramdas,DN Stivers,DM Jones,HM Kantarjian,EH Estey,S Vadhan-Raj,LJ Medeiros,CE Bueso-Ramos,

For similar proteins: dna-binding proteins: nf-kappa b: transcription factor rela research abstracts see: proteins: dna-binding proteins: nf-kappa b: transcription factor rela research

PUBMED ID PMID:

MEDLINE DATE:

Comparative analysis of genes regulated in acute myelomonocytic leukemia with and without inv(16)(p13q22) using microarray techniques, real-time PCR, immunohistochemistry, and flow cytometry immunophenotyping. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Modern pathology : an official journal of the Unit

VOLUME: 20

Page Numbers: 811-20

Journal Abbreviation: Mod. Pathol.

ISSN: 0893-3952

DAY: 15

MONTH: 06

YEAR: 2007

Comparative analysis of genes regulated in acute myelomonocytic leukemia with and without inv(16)(p13q22) using microarray techniques, real-time PCR, immunohistochemistry, and flow cytometry immunophenotyping. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8806605

Comparative analysis of genes regulated in acute myelomonocytic leukemia with and without inv(16)(p13q22) using microarray techniques, real-time PCR, immunohistochemistry, and flow cytometry immunophenotyping. Keywords Mesh Terms:

KEYWORDS: Transcription Factor RelA

MESH TERMS: analysis

Chemical & Substance for Abstract: Comparative analysis of genes regulated in acute myelomonocytic leukemia with and without inv(16)(p13q22) using microarray techniques, real-time PCR, immunohistochemistry, and flow cytometry immunophenotyping. Information

Substance Name: Transcription Factor RelA

Registry Number: 0

Grant and Affiliation Information for Comparative analysis of genes regulated in acute myelomonocytic leukemia with and without inv(16)(p13q22) using microarray techniques, real-time PCR, immunohistochemistry, and flow cytometry immunophenotyping.

AFFILIATION: Division of Pathology and Laboratory Medicine, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.

Country: United States

AGENCY: United States NCI

GRANT: 5P30 CA016672-28

ACRONYM: CA

MEDLINETA: Mod Pathol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Comparative analysis of genes regulated in acute myelomonocytic leukemia with and without inv16p13q22 using microarray techniques, real-time PCR, immunohistochemistry, and flow cytometry immunophenotyping Related Publications

• Comparative analysis of genes regulated in acute myelomonocytic leukemia with and without inv(16)(p13q22) using microarray techniques, real-time PCR, immunohistochemistry, and flow cytometry immunophenotyping.
• Epstein-Barr virus-associated, CD20- polyclonal lymphoproliferative disorder after matched unrelated donor marrow transplantation.