Community analysis of ammonia oxidizer in the oxygen-limited nitritation stage of OLAND system by DGGE of PCR amplified 16S rDNA fragments and FISH.

Community analysis of ammonia oxidizer in the oxygen-limited nitritation stage of OLAND system by DGGE of PCR amplified 16S rDNA fragments and FISH. Research Abstract Details 

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Community analysis of ammonia oxidizer in the oxygen-limited nitritation stage of OLAND system by DGGE of PCR amplified 16S rDNA fragments and FISH. Abstract Text:

Dan Zhang,De-Min Zhang,Yao-Ping Liu,Wen-Wei Cao,Guan-Xiong Chen,

OLAND (oxygen limited autotrophic nitrification and denitrification) nitrogen removal system was constructed by coupling with oxygen limited nitritation stage and anaerobic ammonium oxidation stage. Ammonia oxidizer, as a kind of key bacteria in N cycle, plays an important role at the oxygen limited nitritation stage of OLAND nitrogen removal system. In this study, specific amplification of 16S rDNA fragment of ammonia oxidizer by nested PCR, separation of mixed PCR samples by denaturing gradient gel electrophoresis (DGGE), and the quantification of ammonia oxidizer by fluorescence in situ hybridization (FISH) were combined to investigate the shifts of community composition and quantity of ammonia oxidizer of the oxygen limited nitritation stage in OLAND system. It showed that the community composition of ammonia oxidizer changed drastically when dissolved oxygen was decreased gradually, and the dominant ammonia oxidizer of the steady nitrite accumulation stage were completely different from that of the early stage of oxygen limited nitritation identified by DGGE. It was concluded that the Nitrosomonas may be the dominant genus of ammonia oxidizer at the oxygen limited nitritation stage of OLAND system characterized by nested PCR-DGGE and FISH, and the percentage of Nitrosomonas was 72.5% +/- 0.8% of ammonia oxidizer at the steady nitrite accumulation stage detected by FISH.

Community analysis of ammonia oxidizer in the oxygen-limited nitritation stage of OLAND system by DGGE of PCR amplified 16S rDNA fragments and FISH. Publishing Authors By Initials

D Zhang,DM Zhang,YP Liu,WW Cao,GX Chen,

For similar waste disposal, fluid research abstracts see: waste disposal, fluid research

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Community analysis of ammonia oxidizer in the oxygen-limited nitritation stage of OLAND system by DGGE of PCR amplified 16S rDNA fragments and FISH. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of environmental sciences (China)

VOLUME: 16

Page Numbers: 838-42

Journal Abbreviation: J Environ Sci (China)

ISSN: 1001-0742

DAY: 8

MONTH: 02

YEAR: 2004

Community analysis of ammonia oxidizer in the oxygen-limited nitritation stage of OLAND system by DGGE of PCR amplified 16S rDNA fragments and FISH. Information

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LANGUAGE: eng

NlmUniqueID: 100967627

Community analysis of ammonia oxidizer in the oxygen-limited nitritation stage of OLAND system by DGGE of PCR amplified 16S rDNA fragments and FISH. Keywords Mesh Terms:

KEYWORDS: Waste Disposal, Fluid

MESH TERMS: methods

Chemical & Substance for Abstract: Community analysis of ammonia oxidizer in the oxygen-limited nitritation stage of OLAND system by DGGE of PCR amplified 16S rDNA fragments and FISH. Information

Substance Name: Oxygen

Registry Number: 7782-44-7

Grant and Affiliation Information for Community analysis of ammonia oxidizer in the oxygen-limited nitritation stage of OLAND system by DGGE of PCR amplified 16S rDNA fragments and FISH.

AFFILIATION: Department of Biology, Liaoning Normal University, Dalian 116021, China. zhangdanangel@hotmail.com

Country: Netherlands

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MEDLINETA: J Environ Sci (China)

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