[Common problems in the analyses of microbial community by denaturing gradient gel electrophoresis (DGGE)]

[Common problems in the analyses of microbial community by denaturing gradient gel electrophoresis (DGGE)] Research Abstract Details 

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[Common problems in the analyses of microbial community by denaturing gradient gel electrophoresis (DGGE)] Abstract Text:

De-Feng Xing,Nan-Qi Ren,

Denaturing gradient gel electrophoresis (DGGE) can describe microbial communities based on partial sequences of nucleic acid and monitor uncultured bacterium and it's functional genes. It is well-established molecular tools in microbial molecular ecology that allows the study of diversity and dynamics of microbial communities. Here, common issues in the analyses of microbial community by DGGE were raised, and the feasible resolving methods were introduced in this paper. The effects of samples pretreatment and PCR amplification on DGGE analysis were evaluated. Optimization and statistical analysis of DGGE profiles were discussed. Finally, prospect of DGGE for understanding microbial communities were reviewed.

[Common problems in the analyses of microbial community by denaturing gradient gel electrophoresis (DGGE)] Publishing Authors By Initials

DF Xing,NQ Ren,

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[Common problems in the analyses of microbial community by denaturing gradient gel electrophoresis (DGGE)] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Wei sheng wu xue bao = Acta microbiologica Sinica

VOLUME: 46

Page Numbers: 331-5

Journal Abbreviation: Wei Sheng Wu Xue Bao

ISSN: 0001-6209

DAY: 1

MONTH: Apr

YEAR: 2006

[Common problems in the analyses of microbial community by denaturing gradient gel electrophoresis (DGGE)] Information

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LANGUAGE: chi

NlmUniqueID: 21610860

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AFFILIATION: School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China. ixdf@yahoo.com.cn

Country: China

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MEDLINETA: Wei Sheng Wu Xue Bao

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