[Combined detection of K-ras mutation and CA19-9 level in plasma of patients with pancreatic cancer]

[Combined detection of K-ras mutation and CA19-9 level in plasma of patients with pancreatic cancer] Research Abstract Details 

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[Combined detection of K-ras mutation and CA19-9 level in plasma of patients with pancreatic cancer] Abstract Text:

Meng-hua Dai,Yu-pei Zhao,Li-xing Cai,Yu Zhu,

OBJECTIVE: To confirm the effect of combined detection of K-ras gene mutation and CA19-9 level in plasma in the diagnosis of pancreatic carcinoma. METHODS: In 33 patients without pancreatic carcinoma and 15 patients with pancreatic carcinoma, the value of K-ras mutation and CA19-9 level were examined by enriched polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP) and radioimmunoassay respectively. The data were analysed with chi(2) test, Fisher's exact test and Kruekal-wall's test. All patients were confirmed pathologically. RESULTS: Eleven of the 15 patients with pancreatic carcinoma and 2 of 10 patients with chronic pancreatitis were found K-ras mutation. CA19-9 level in patients with pancreatic cancer was significantly higher than that in those with benign disease but with a low specificity. The sensitivity, specificity of combined detection of K-ras gene mutation and CA19-9 level in the plasma of patients with pancreatic cancer were 66.67% and 97%. There was a significant difference between patients with pancreatic cancer and those without pancreatic cancer. Pathological examination showed atypical hyperplasia in one patient with chronic pancreatitis who had K-ras mutation and higher CA19-9 level. CONCLUSIONS: Combined detection of K-ras gene mutation and CA19-9 can be used for adjuvant diagnosis of pancreatic cancer. It is more specific than detection of single K-ras gene mutation or CA19-9 level in the plasma. The method is complementary to single detection of K-ras gene mutation or CA19-9 level because it decreases the rate of false-positive diagnosis.

[Combined detection of K-ras mutation and CA19-9 level in plasma of patients with pancreatic cancer] Publishing Authors By Initials

MH Dai,YP Zhao,LX Cai,Y Zhu,

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[Combined detection of K-ras mutation and CA19-9 level in plasma of patients with pancreatic cancer] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Zhonghua wai ke za zhi [Chinese journal of surgery

VOLUME: 41

Page Numbers: 332-5

Journal Abbreviation: Zhonghua Wai Ke Za Zhi

ISSN: 0529-5815

DAY: 1

MONTH: May

YEAR: 2003

[Combined detection of K-ras mutation and CA19-9 level in plasma of patients with pancreatic cancer] Information

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LANGUAGE: chi

NlmUniqueID: 153611

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Grant and Affiliation Information for [Combined detection of K-ras mutation and CA19-9 level in plasma of patients with pancreatic cancer]

AFFILIATION: Department of General Surgery. Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China.

Country: China

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MEDLINETA: Zhonghua Wai Ke Za Zhi

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