Combined atomic force microscopy and optical microscopy measurements as a method to investigate particle uptake by cells.

Combined atomic force microscopy and optical microscopy measurements as a method to investigate particle uptake by cells. Research Abstract Details 

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Combined atomic force microscopy and optical microscopy measurements as a method to investigate particle uptake by cells. Abstract Text:

We propose a combination of atomic force microscopy (AFM) and optical microscopy for the investigation of particle uptake by cells. Positively and negatively charged polymer microcapsules were chosen as model particles, because their interaction with cells had already been investigated in detail. AFM measurements allowed the recording of adhesion forces on a single-molecule level. Due to the micrometer size of the capsules, the number of ingested capsules could be counted by optical microscopy. The combination of both methods allowed combined measurement of the adhesion forces and the uptake rate for the same model particle. As a demonstration of this system, the correlation between the adhesion of positively or negatively charged polymer microcapsules onto cell surfaces and the uptake of these microcapsules by cells has been investigated for several cell lines. As is to be expected, we find a correlation between both processes, which is in agreement with adsorption-dependent uptake of the polymer microcapsules by cells.

Combined atomic force microscopy and optical microscopy measurements as a method to investigate particle uptake by cells. Publishing Authors By Initials

For similar macromolecular substances: polymers research abstracts see: macromolecular substances: polymers research

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Combined atomic force microscopy and optical microscopy measurements as a method to investigate particle uptake by cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Small (Weinheim an der Bergstrasse, Germany)

VOLUME: 2

Page Numbers: 394-400

Journal Abbreviation: Small

ISSN: 1613-6829

DAY: 17

MONTH: Mar

YEAR: 2006

Combined atomic force microscopy and optical microscopy measurements as a method to investigate particle uptake by cells. Information

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LANGUAGE: eng

NlmUniqueID: 101235338

Combined atomic force microscopy and optical microscopy measurements as a method to investigate particle uptake by cells. Keywords Mesh Terms:

KEYWORDS: Polymers

MESH TERMS: pharmacokinetics

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Substance Name: Polymers

Registry Number: 0

Grant and Affiliation Information for Combined atomic force microscopy and optical microscopy measurements as a method to investigate particle uptake by cells.

AFFILIATION: Physics Department and Center for Nanoscience, Ludwig Maximilians Universität München, 80799 Munich, Germany.

Country: Germany

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MEDLINETA: Small

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