[Combination radiation and gene therapy for head and neck squamous cell carcinoma in the murine model]

[Combination radiation and gene therapy for head and neck squamous cell carcinoma in the murine model] Research Abstract Details 

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[Combination radiation and gene therapy for head and neck squamous cell carcinoma in the murine model] Abstract Text:

Shi-xi Liu,Huan Yang,Yi-ming Yuan,Yu-fen Guo,Zhi-quan Tang,Chuan-yu Liang,

OBJECTIVE: To examine the antitumour efficacy and investigate immunological mechanism of combination therapy of IL-2 gene and IL-12 gene transfer with radiation in an immunocompetent murine model that parallel more closely the clinical therapy of head and neck squamous cell carcinoma (HNSCC). METHODS: Tumors were established in the floor of mouth in C3H/HeJ mice with SCCVII cell line. Lipid-DNA complexed (lipoplexes) by using polycationic liposome-Mediated transduction for HNSCC was transducted in tumor-bearing mouse by direct intratumoral gene transfer. The local tumor was radiated with a dose of 2 Gy in the second day. Tumor sizes were measured before and after the treatment as compared to the different single treatment groups and the controls. After tumors were subculture, the supernatants were collected for IL-2 and IL-12 expression by enzyme-linked immunosobent assay (ELISA). Natural killer (NK) cell activity and cytotoxic T-lymphocyte (CTL) activity were also assayed by LDH methed. CD4+ and CD8+ T-lymphocyte in tumor tissues were examined by immunohistochemistry. RESULTS: HNSCC tumor growth was significantly inhibited following comined IL-2 and IL-12 gene therapy with radiation as compared to IL-2 or IL-12 gene therapy with radiation, single IL-2 or IL-12 gene therapy, radiation alone and the controls. Increased secreted levels of IL-2 and IL-12 protein expression were found in combined and single IL-2 gene or IL-12 gene treated groups. The combination and single gene treated groups produced greater activation of CTL and NK than the controls of all concerned test. The significant CD4+ and CD8+ T lymphocyte infiltration was distributed and the numerous necrosis were seen in tumor tissues after combination therapy. CONCLUSIONS: Combined IL-2 gene and IL-12 gene therapy with radiation could significantly inhibited HNSCC tumor growth in the murine model and efficiently induced antitumor immnunity of the host.

[Combination radiation and gene therapy for head and neck squamous cell carcinoma in the murine model] Publishing Authors By Initials

SX Liu,H Yang,YM Yuan,YF Guo,ZQ Tang,CY Liang,

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[Combination radiation and gene therapy for head and neck squamous cell carcinoma in the murine model] Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Zhonghua er bi yan hou ke za zhi

VOLUME: 39

Page Numbers: 278-82

Journal Abbreviation: Zhonghua Er Bi Yan Hou Ke Za Z

ISSN: 0412-3948

DAY: 1

MONTH: May

YEAR: 2004

[Combination radiation and gene therapy for head and neck squamous cell carcinoma in the murine model] Information

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LANGUAGE: chi

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AFFILIATION: Department of Otorhinolaryngology, West China Hospital, Sichuan University, Chengdu 610041, China. liusx@mcwcums.com

Country: China

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MEDLINETA: Zhonghua Er Bi Yan Hou Ke Za Z

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