Colorimetric assay of propranolol tablets by derivatization: novel application of diazotized 4-amino-3,5-dinitrobenzoic acid (ADBA).

Colorimetric assay of propranolol tablets by derivatization: novel application of diazotized 4-amino-3,5-dinitrobenzoic acid (ADBA). Research Abstract Details 

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Colorimetric assay of propranolol tablets by derivatization: novel application of diazotized 4-amino-3,5-dinitrobenzoic acid (ADBA). Abstract Text:

A novel colorimetric assay of propranolol tablets has been developed. The assay is based on chemical derivatization (aromatic ring derivatization technique) using diazotized 4-amino-3,5-dinitrobenzoic acid (ADBA) as the chromogenic derivatizing reagent and resultant formation of azo dyes. Optimization studies established an optimal reaction time of 5 min at 30 degrees C after mixing on a Vortex mixer the drug/reagent mixture for 10 s. A new absorption maximum (lambda(max)) was found at 470 nm, which was selected as the analytical wavelength. The assays were linear over 1-8 microg/mL propranolol, and the reaction occurred by a 1:1 reagent/drug stoichiometric ratio. The developed method has a low limit of detection of 0.76 microg/mL and is reproducible. It has been applied successfully to the assay of propranolol tablets and is of equivalent accuracy (p > 0.05) with the official (British Pharmacopoeia) ultraviolet spectrophotometric method. The new method has the main advantage of using more affordable instrumentation and could be applied to the in-process quality control of propranolol tablets.

Colorimetric assay of propranolol tablets by derivatization: novel application of diazotized 4-amino-3,5-dinitrobenzoic acid (ADBA). Publishing Authors By Initials

For similar pharmaceutical preparations: dosage forms: tablets research abstracts see: pharmaceutical preparations: dosage forms: tablets research

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Colorimetric assay of propranolol tablets by derivatization: novel application of diazotized 4-amino-3,5-dinitrobenzoic acid (ADBA). Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of AOAC International

VOLUME: 87

Page Numbers: 573-8

Journal Abbreviation: J AOAC Int

ISSN: 1060-3271

DAY: 17

MONTH: 03

YEAR: 2008

Colorimetric assay of propranolol tablets by derivatization: novel application of diazotized 4-amino-3,5-dinitrobenzoic acid (ADBA). Information

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LANGUAGE: eng

NlmUniqueID: 9215446

Colorimetric assay of propranolol tablets by derivatization: novel application of diazotized 4-amino-3,5-dinitrobenzoic acid (ADBA). Keywords Mesh Terms:

KEYWORDS: Tablets

MESH TERMS: analysis

Chemical & Substance for Abstract: Colorimetric assay of propranolol tablets by derivatization: novel application of diazotized 4-amino-3,5-dinitrobenzoic acid (ADBA). Information

Substance Name: Propranolol

Registry Number: 525-66-6

Grant and Affiliation Information for Colorimetric assay of propranolol tablets by derivatization: novel application of diazotized 4-amino-3,5-dinitrobenzoic acid (ADBA).

AFFILIATION: University of Ibadan, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Orita UI, Ibadan-Oyo Rd, Nigeria. olakunleid@yahoo.com

Country: United States

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MEDLINETA: J AOAC Int

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