Collagen IV induces trophoectoderm differentiation of mouse embryonic stem cells.

Collagen IV induces trophoectoderm differentiation of mouse embryonic stem cells. Research Abstract Details 

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Collagen IV induces trophoectoderm differentiation of mouse embryonic stem cells. Abstract Text:

Katja Schenke-Layland,Ekaterini Angelis,Katrin E Rhodes,Sepideh Heydarkhan-Hagvall,Hanna K Mikkola,W Robb Maclellan,

The earliest segregation of lineages in the developing embryo is the commitment of cells to the inner cell mass or the trophoectoderm in preimplantation blastocysts. The exogenous signals that control commitment to a particular cell lineage are poorly understood; however, it has been suggested that extracellular "niche" and extracellular matrix, in particular, play an important role in determining the developmental fate of stem cells. Collagen IV (ColIV) has been reported to direct embryonic stem (ES) cell differentiation to mesodermal lineages in both mouse and human ES cells. To define the effects of ColIV on ES cell differentiation and to identify the resulting heterogeneous cell types, we performed microarray analyses and determined global gene expression. We observed that ColIV induced the expression of mesodermal genes specific to hematopoietic, endothelial, and smooth muscle cells and, surprisingly, also a panel of trophoectoderm-restricted markers. This effect was specific to collagen IV, as no trophoblast differentiation was seen on collagen I, laminin, or fibronectin. Stimulation with basic fibroblast growth factor (FGF) or FGF4 increased the number of trophoectodermal cells. These cells were isolated under clonal conditions and successfully differentiated into a variety of trophoblast derivatives. Interestingly, differentiation of ES cells to trophoblastic lineages was only seen in ES cell lines maintained on embryonic feeder layers and was caudal-type homeobox protein 2 (Cdx2)-dependent, consistent with Cdx2's postulated role in trophoectoderm commitment. Our data suggest that, given the appropriate extracellular stimuli, mouse embryonic stem cells can differentiate into trophoectoderm. Disclosure of potential conflicts of interest is found at the end of this article.

Collagen IV induces trophoectoderm differentiation of mouse embryonic stem cells. Publishing Authors By Initials

K Schenke-Layland,E Angelis,KE Rhodes,S Heydarkhan-Hagvall,HK Mikkola,WR Maclellan,

For similar cells: trophoblasts research abstracts see: cells: trophoblasts research

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Collagen IV induces trophoectoderm differentiation of mouse embryonic stem cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Stem cells (Dayton, Ohio)

VOLUME: 25

Page Numbers: 1529-38

Journal Abbreviation: Stem Cells

ISSN: 1066-5099

DAY: 15

MONTH: 03

YEAR: 2007

Collagen IV induces trophoectoderm differentiation of mouse embryonic stem cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9304532

Collagen IV induces trophoectoderm differentiation of mouse embryonic stem cells. Keywords Mesh Terms:

KEYWORDS: Trophoblasts

MESH TERMS: drug effects

Chemical & Substance for Abstract: Collagen IV induces trophoectoderm differentiation of mouse embryonic stem cells. Information

Substance Name: Fibroblast Growth Factors

Registry Number: 62031-54-3

Grant and Affiliation Information for Collagen IV induces trophoectoderm differentiation of mouse embryonic stem cells.

AFFILIATION: Cardiovascular Research Laboratory, UCLA School of Medicine, 675 C.E. Young Dr., MRL 3-645, Los Angeles, California 90095-1760, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: R21DK069659

ACRONYM: DK

MEDLINETA: Stem Cells

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