Collagen degradation induced by the combination of IL-1alpha and plasminogen in rabbit articular cartilage explant culture.

Collagen degradation induced by the combination of IL-1alpha and plasminogen in rabbit articular cartilage explant culture. Research Abstract Details 

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Collagen degradation induced by the combination of IL-1alpha and plasminogen in rabbit articular cartilage explant culture. Abstract Text:

S Saito,M Katoh,M Masumoto,S Matsumoto,Y Masuho,

To investigate the effect of plasminogen on cartilage catabolism, we assessed collagen degradation in rabbit articular cartilage explants treated with or without plasminogen and interleukin-1alpha (IL-1alpha). The combination of IL-1 alpha and plasminogen induced rapid collagen degradation, amounting to more than 60% of the total collagen by day 7, while neither IL-1alpha nor plasminogen alone had any effect. To examine the mechanism of collagen degradation induced by IL-1alpha and plasminogen, the matrix metalloproteinases (MMPs) in the culture supernatants were examined by ELISA, Western blotting and gelatin zymography. We found that the treatment with IL-1alpha induced MMP-1, MMP-3, and MMP-9. In addition, plasminogen converted the pro form of MMPs into the active form. Both a tissue inhibitor of metalloproteinases-1 (TIMP-1) and a synthetic hydroxamate MMP inhibitor prevented this collagen release. These results suggest that plasminogen causes collagen degradation via activation of MMPs induced by IL-1alpha.

Collagen degradation induced by the combination of IL-1alpha and plasminogen in rabbit articular cartilage explant culture. Publishing Authors By Initials

S Saito,M Katoh,M Masumoto,S Matsumoto,Y Masuho,

For similar proteins: tissue inhibitor of metalloproteinases research abstracts see: proteins: tissue inhibitor of metalloproteinases research

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Collagen degradation induced by the combination of IL-1alpha and plasminogen in rabbit articular cartilage explant culture. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 122

Page Numbers: 49-54

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jul

YEAR: 1997

Collagen degradation induced by the combination of IL-1alpha and plasminogen in rabbit articular cartilage explant culture. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Collagen degradation induced by the combination of IL-1alpha and plasminogen in rabbit articular cartilage explant culture. Keywords Mesh Terms:

KEYWORDS: Tissue Inhibitor of Metalloproteinases

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Collagen degradation induced by the combination of IL-1alpha and plasminogen in rabbit articular cartilage explant culture. Information

Substance Name: Matrix Metalloproteinase 1

Registry Number: EC 3.4.24.7

Grant and Affiliation Information for Collagen degradation induced by the combination of IL-1alpha and plasminogen in rabbit articular cartilage explant culture.

AFFILIATION: Institute for Drug Discovery Research, Yamanouchi Pharmaceutical Co., Ltd., Ibaraki.

Country: JAPAN

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MEDLINETA: J Biochem

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