Cold storage of mouse embryos of different stages of development.

Cold storage of mouse embryos of different stages of development. Research Abstract Details 

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Cold storage of mouse embryos of different stages of development. Abstract Text:

C M Herr,R W Wright,

Experiments were designed to evaluate the survival rates of preimplantation mouse embryos of different stages of development in cold culture at 4 degrees C. Several developmental stages, from one-cell to the blastocyst, were stored at 4 degrees C from 1 to 8 d. Viability following cold culture was determined by blastocyst expansion during culture in Whitten's medium at 37 degrees C. Blastocyst formation of nonstored controls ranged from 93 to 100% for all developmental stages tested. Only 3% of one-cell embryos survived 1 d and none survived 2 days at 4 degrees C. Survival improved using two-cell embryos, with 84, 69 and 15% forming expanded blastocysts following storage for 1, 2 and 3 d, respectively. Eighty five and 38% of eight-cell embryos formed expanded blastocysts following cold storage for 3 and 4 d, respectively. Survival rates for cold stored morulae and blastocysts remained above 75% for 6 d but decreased significantly to 30 and 36%, respectively, when stored for 8 d. A large percentage of blastocysts were observed to collapse when placed in cold storage from 1 to 8 d but almost all expanded when placed in culture at 37 degrees C. This study showed that one-cell embryos were particularly sensitive to cold storage compared to later-stage mouse embryos. Cold storage survival increased with increasing age of the embryo; morula and blastocyst survival rate was similar.

Cold storage of mouse embryos of different stages of development. Publishing Authors By Initials

CM Herr,RW Wright,

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Cold storage of mouse embryos of different stages of development. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Theriogenology

VOLUME: 29

Page Numbers: 765-70

Journal Abbreviation: Theriogenology

ISSN: 0093-691X

DAY: 26

MONTH: 05

YEAR: 1988

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LANGUAGE: eng

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Country: United States

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MEDLINETA: Theriogenology

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