Coimmobilization of dehydrogenases and their cofactors in electrochemical biosensors.

Coimmobilization of dehydrogenases and their cofactors in electrochemical biosensors. Research Abstract Details 

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Coimmobilization of dehydrogenases and their cofactors in electrochemical biosensors. Abstract Text:

Maogen Zhang,Conor Mullens,Waldemar Gorski,

Enzyme-based reagentless biosensors were developed using the model system of glucose dehydrogenase (GDH) and its nicotinamide adenine dinucleotide cofactor (NAD+). The biosensors were prepared following an approach similar to the concept of molecular imprinting. To this end, the N1-carboxymethyl-NAD+ species were covalently attached to polyamino-saccharide chains of chitosan (CHIT) and allowed to interact with GDH in an aqueous solution. The bioaffinity interactions between the NAD+ and GDH were secured by cross-linking the system with the glutaric dialdehyde (GDI)-modified CHIT. Electron conductive films of such CHIT-NAD+-GDH-GDI-CHIT macrocomplexes (MC) were prepared on glassy carbon (GC) electrodes by adding carbon nanotubes (CNT) and evaporating water. Electrochemical analysis of the GC/CNT-MC electrodes revealed that, in contrast to the oxidase-based electrodes, they acted as oxygen-independent reagentless biosensors. The application of Nafion to such biosensors predictably improved their selectivity and, unexpectedly, enhanced their sensitivity by an order of magnitude.

Coimmobilization of dehydrogenases and their cofactors in electrochemical biosensors. Publishing Authors By Initials

M Zhang,C Mullens,W Gorski,

For similar investigative techniques: epidemiologic methods: statistics as topic: sensitivity and specificity research abstracts see: investigative techniques: epidemiologic methods: statistics as topic: sensitivity and specificity research

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MEDLINE DATE:

Coimmobilization of dehydrogenases and their cofactors in electrochemical biosensors. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Analytical chemistry

VOLUME: 79

Page Numbers: 2446-50

Journal Abbreviation: Anal. Chem.

ISSN: 0003-2700

DAY: 14

MONTH: 02

YEAR: 2007

Coimmobilization of dehydrogenases and their cofactors in electrochemical biosensors. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370536

Coimmobilization of dehydrogenases and their cofactors in electrochemical biosensors. Keywords Mesh Terms:

KEYWORDS: Sensitivity and Specificity

MESH TERMS: metabolism

Chemical & Substance for Abstract: Coimmobilization of dehydrogenases and their cofactors in electrochemical biosensors. Information

Substance Name: Oxidoreductases

Registry Number: EC 1.-

Grant and Affiliation Information for Coimmobilization of dehydrogenases and their cofactors in electrochemical biosensors.

AFFILIATION: Department of Chemistry, University of Texas at San Antonio, San Antonio, Texas 78249-0698, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM 08194

ACRONYM: GM

MEDLINETA: Anal Chem

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