Cognate putative nuclear localization signal effects strong nuclear localization of a GFP reporter and facilitates gene expression studies in Caenorhabditis elegans.

Cognate putative nuclear localization signal effects strong nuclear localization of a GFP reporter and facilitates gene expression studies in Caenorhabditis elegans. Research Abstract Details 

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Cognate putative nuclear localization signal effects strong nuclear localization of a GFP reporter and facilitates gene expression studies in Caenorhabditis elegans. Abstract Text:

Nicholas N Lyssenko,Wendy Hanna-Rose,Robert A Schlegel,Nicholas N Lyssenko,Wendy Hanna-Rose,Robert A Schlegel,

Targeting a gene expression reporter, usually the green fluorescent protein (GFP), to the nucleus via a translationally fused nuclear localization signal (NLS) greatly facilitates recognition and identification of the reporter-expressing cells in Caenorhabditis elegans. Presently circulating nematode transcriptional gene expression vectors use the viral NLS from simian virus 40 (SV40) large T antigen. This NLS, however fails to ensure sufficient localization of the GFP peptide to the nucleus. We modified the common transcriptional reporter SV40 NLS-GFP by adding to its C terminus a cognate putative NLS from the transcription factor egl-13. The EGL-13 NLS effected clear contrast in fluorescence intensity between the nucleus and the cytoplasm in cells with strong reporter signal and efficiently highlighted the nucleus in tissues with weak reporter expression in a wide range of tested tissues. The SV40 NLS-GFP-EGL-13 NLS vector should become a valuable tool for gene expression studies in C. elegans.

Cognate putative nuclear localization signal effects strong nuclear localization of a GFP reporter and facilitates gene expression studies in Caenorhabditis elegans. Publishing Authors By Initials

NN Lyssenko,W Hanna-Rose,RA Schlegel,NN Lyssenko,W Hanna-Rose,RA Schlegel,

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Cognate putative nuclear localization signal effects strong nuclear localization of a GFP reporter and facilitates gene expression studies in Caenorhabditis elegans. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: BioTechniques

VOLUME: 43

Page Numbers: 596, 598, 560

Journal Abbreviation: BioTechniques

ISSN: 0736-6205

DAY: 12

MONTH: Nov

YEAR: 2007

Cognate putative nuclear localization signal effects strong nuclear localization of a GFP reporter and facilitates gene expression studies in Caenorhabditis elegans. Information

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LANGUAGE: eng

NlmUniqueID: 8306785

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Grant and Affiliation Information for Cognate putative nuclear localization signal effects strong nuclear localization of a GFP reporter and facilitates gene expression studies in Caenorhabditis elegans.

AFFILIATION: The Pennsylvania State University, University Park, PA, USA. lyssenn@ccf.org

Country: England

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MEDLINETA: Biotechniques

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