Coexpression of the CUG-binding protein reduces DM protein kinase expression in COS cells.

Coexpression of the CUG-binding protein reduces DM protein kinase expression in COS cells. Research Abstract Details 

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Coexpression of the CUG-binding protein reduces DM protein kinase expression in COS cells. Abstract Text:

N Takahashi,N Sasagawa,F Usuki,Y Kino,H Kawahara,H Sorimachi,T Maeda,K Suzuki,S Ishiura,

Myotonic dystrophy (DM) is the most common form of adult onset muscular dystrophy. Patients have a large CTG repeat expansion in the 3' untranslated region of the DMPK gene, which encodes DM protein kinase. RNA trans-dominant models, which hypothesize that the expanded CUG trinucleotide repeat on DMPK mRNA sequesters a factor or disrupts the RNA metabolism of the DMPK mRNA itself and other mRNAs in a trans dominant manner, have been proposed. A candidate for the sequestered factor, termed CUG-binding protein (CUG-BP), exists in several alternatively spliced isoforms. We found a human isoform with a twelve base insertion (deduced amino acids Leu-Tyr-Leu-Gln) and an isoform with a three base insertion (deduced amino acid Ala) insertion. In order to elucidate the effects of CUG-BP on DMPK expression, we introduced CUG-BP and DMPK cDNA transiently into COS-7 cells. Cotransfection of CUG-BP did not significantly affect the expression of either wild type or mutant DMPK at the mRNA level. On the other hand, cotransfection of CUG-BP significantly affected the expression of both the wild type and mutant DMPKs at the protein level. This reduction was remarkable when the mutant DMPK construct was used.

Coexpression of the CUG-binding protein reduces DM protein kinase expression in COS cells. Publishing Authors By Initials

N Takahashi,N Sasagawa,F Usuki,Y Kino,H Kawahara,H Sorimachi,T Maeda,K Suzuki,S Ishiura,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: molecular structure: base sequence: repetitive sequences, nucleic acid: tandem repeat sequences: microsatellite repeats: trinucleotide repeats research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: molecular structure: base sequence: repetitive sequences, nucleic acid: tandem repeat sequences: microsatellite repeats: trinucleotide repeats research

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Coexpression of the CUG-binding protein reduces DM protein kinase expression in COS cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 130

Page Numbers: 581-7

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Nov

YEAR: 2001

Coexpression of the CUG-binding protein reduces DM protein kinase expression in COS cells. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Coexpression of the CUG-binding protein reduces DM protein kinase expression in COS cells. Keywords Mesh Terms:

KEYWORDS: Trinucleotide Repeats

MESH TERMS: genetics

Chemical & Substance for Abstract: Coexpression of the CUG-binding protein reduces DM protein kinase expression in COS cells. Information

Substance Name: Protein-Serine-Threonine Kinases

Registry Number: EC 2.7.11.1

Grant and Affiliation Information for Coexpression of the CUG-binding protein reduces DM protein kinase expression in COS cells.

AFFILIATION: Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro-ku, Tokyo 153-8902, Japan.

Country: Japan

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MEDLINETA: J Biochem

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