[Coding single nucleotide polymorphism is an ideal marker for detecting gene imprinting by 5' nuclease assay]

[Coding single nucleotide polymorphism is an ideal marker for detecting gene imprinting by 5' nuclease assay] Research Abstract Details 

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[Coding single nucleotide polymorphism is an ideal marker for detecting gene imprinting by 5' nuclease assay] Abstract Text:

Mo-bin Wan,Guan-shan Zhu,Rui-ying Zheng,

OBJECTIVE: To establish a novel approach for quick and high throughput verification of human gene imprinting. METHODS: By use of a pair of dye-labeled probes, 5' nuclease assay was combined with reverse transcriptase-PCR(RT-PCR) to genotype a coding single nucleotide polymorphism (cSNP), rs705(C/T) of a known imprinted gene, small nuclear ribonucleotide protein N (SNRPN), on both genomic DNA and cDNA of human lymphoblast cell lines. RESULTS: Allele discrimination showed a clear monoallelic expression pattern of SNRPN, which was confirmed by RT-PCR based restriction fragment length polymorphisms. Pedigree analysis verified the paternal origin of expressed allele, which is in consistency with previous report. CONCLUSION: Coding SNP is an ideal marker for detecting gene imprinting by 5' nuclease assay. This approach has also a potentiality to discover differential allele expression of non-imprinted genes in order to find gene cis-acting functional polymorphism.

[Coding single nucleotide polymorphism is an ideal marker for detecting gene imprinting by 5' nuclease assay] Publishing Authors By Initials

MB Wan,GS Zhu,RY Zheng,

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[Coding single nucleotide polymorphism is an ideal marker for detecting gene imprinting by 5' nuclease assay] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Zhonghua yi xue yi chuan xue za zhi = Zhonghua yix

VOLUME: 20

Page Numbers: 225-7

Journal Abbreviation: Zhonghua Yi Xue Yi Chuan Xue Z

ISSN: 1003-9406

DAY: 2

MONTH: Jun

YEAR: 2003

[Coding single nucleotide polymorphism is an ideal marker for detecting gene imprinting by 5' nuclease assay] Information

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LANGUAGE: chi

NlmUniqueID: 9425197

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Grant and Affiliation Information for [Coding single nucleotide polymorphism is an ideal marker for detecting gene imprinting by 5' nuclease assay]

AFFILIATION: Department of Infectious Diseases, Changhai Hospital, Second Military Medical University, Shanghai, 200433 PR China. wanmb@online.sh.cn

Country: China

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MEDLINETA: Zhonghua Yi Xue Yi Chuan Xue Z

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