Codeine induces human mast cell chemokine and cytokine production: involvement of G-protein activation.

Codeine induces human mast cell chemokine and cytokine production: involvement of G-protein activation. Research Abstract Details 

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Codeine induces human mast cell chemokine and cytokine production: involvement of G-protein activation. Abstract Text:

C H Sheen,R P Schleimer,M Kulka,C H Sheen,R P Schleimer,M Kulka,

BACKGROUND: Activation of mast cells and the systemic release of histamine are common side effects of opiates such as codeine and morphine. In some individuals, codeine not only elicits a sizable early response due to mast cell degranulation, but can also lead to late cutaneous allergic inflammation possibly through the production of chemokines. However, individuals who exhibit a late phase reaction to codeine often do not react to its synthetic analog, meperidine. The goal of this study was to test whether codeine and meperidine induce secretion of inflammatory mediators in human mast cells. METHODS: To characterize opiate activation of human mast cells, we stimulated cultured human (LAD2 cell line and CD34+-derived) mast cells with codeine and meperidine and measured degranulation and chemokine production. RESULTS: Codeine, but not meperidine, activated human mast cell degranulation within 30 min in a dose-dependent manner. Degranulation was blocked by the phosphoinositol-3 kinase (PI3K) inhibitor, wortmannin, and pertussis toxin but not by Ro-31-8220, a PKC inhibitor or forskolin, a cyclic adenylyl cyclase activator. After 3 and 8 h of stimulation, codeine, but not meperidine, activated human mast cells to release monocyte chemoattractant protein-1 (CCL2), regulated on activation, normal T expressed and secreted (RANTES, CCL5) and interleukin-8 (CXCL 8) but not inducible protein-10 (CXCL10). CONCLUSIONS: Codeine activates human mast cell degranulation and chemokine production by activating protein kinase A and PI3 kinase, possibly leading to NF-kappaB activation. Therefore, opiates may regulate late phase allergic inflammation by activating chemokine production by human mast cells.

Codeine induces human mast cell chemokine and cytokine production: involvement of G-protein activation. Publishing Authors By Initials

CH Sheen,RP Schleimer,M Kulka,CH Sheen,RP Schleimer,M Kulka,

For similar cells: connective tissue cells: mast cells research abstracts see: cells: connective tissue cells: mast cells research

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Codeine induces human mast cell chemokine and cytokine production: involvement of G-protein activation. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Allergy

VOLUME: 62

Page Numbers: 532-8

Journal Abbreviation:

ISSN: 0105-4538

DAY: 3

MONTH: May

YEAR: 2007

Codeine induces human mast cell chemokine and cytokine production: involvement of G-protein activation. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7804028

Codeine induces human mast cell chemokine and cytokine production: involvement of G-protein activation. Keywords Mesh Terms:

KEYWORDS: Mast Cells

MESH TERMS: metabolism

Chemical & Substance for Abstract: Codeine induces human mast cell chemokine and cytokine production: involvement of G-protein activation. Information

Substance Name: GTP-Binding Proteins

Registry Number: EC 3.6.1.-

Grant and Affiliation Information for Codeine induces human mast cell chemokine and cytokine production: involvement of G-protein activation.

AFFILIATION: Allergy/Immunology Division, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA.

Country: Denmark

AGENCY: United States NHLBI

GRANT: R01HL078860

ACRONYM: HL

MEDLINETA: Allergy

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