Cobalt chloride, a hypoxia-mimicking agent, targets sterol synthesis in the pathogenic fungus Cryptococcus neoformans.

Cobalt chloride, a hypoxia-mimicking agent, targets sterol synthesis in the pathogenic fungus Cryptococcus neoformans. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Cobalt chloride, a hypoxia-mimicking agent, targets sterol synthesis in the pathogenic fungus Cryptococcus neoformans. Abstract Text:

Hyeseung Lee,Clara M Bien,Adam L Hughes,Peter J Espenshade,Kyung J Kwon-Chung,Yun C Chang,

We investigated the effects of the hypoxia-mimetic CoCl2 in the pathogenic fungus Cryptococcus neoformans and demonstrated that CoCl2 leads to defects in several enzymatic steps in ergosterol biosynthesis. Sterol defects were amplified in cells lacking components of the Sre1p-mediated oxygen-sensing pathway. Consequently, Sre1p and its binding partner Scp1p were essential for growth in the presence of CoCl2. Interestingly, high copies of a single gene involved in ergosterol biosynthesis, ERG25, rescued this growth defect. We show that the inhibitory effect of CoCl2 on scp1Delta and sre1Delta cells likely resulted from either an accumulation of non-viable methylated sterols or a decrease in the amount of ergosterol. Similar findings were also observed in the ascomycetous yeast, Schizosaccharomyces pombe, suggesting that the effects of CoCl2 on the Sre1p-mediated response are conserved in fungi. In addition, gene expression analysis revealed limited overlap between Sre1p-dependant gene activation in the presence of CoCl2 and low oxygen. The majority of genes similarly affected by both CoCl2 and low oxygen were involved in ergosterol synthesis and in iron/copper transport. This article identifies the Sre1p pathway as a common mechanism by which yeast cells sense and adapt to changes in both CoCl2 concentrations and oxygen levels.

Cobalt chloride, a hypoxia-mimicking agent, targets sterol synthesis in the pathogenic fungus Cryptococcus neoformans. Publishing Authors By Initials

H Lee,CM Bien,AL Hughes,PJ Espenshade,KJ Kwon-Chung,YC Chang,

For similar genetic processes: gene expression regulation: up-regulation research abstracts see: genetic processes: gene expression regulation: up-regulation research

PUBMED ID PMID:

MEDLINE DATE:

Cobalt chloride, a hypoxia-mimicking agent, targets sterol synthesis in the pathogenic fungus Cryptococcus neoformans. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Molecular microbiology

VOLUME: 65

Page Numbers: 1018-33

Journal Abbreviation: Mol. Microbiol.

ISSN: 0950-382X

DAY: 21

MONTH: 07

YEAR: 2007

Cobalt chloride, a hypoxia-mimicking agent, targets sterol synthesis in the pathogenic fungus Cryptococcus neoformans. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8712028

Cobalt chloride, a hypoxia-mimicking agent, targets sterol synthesis in the pathogenic fungus Cryptococcus neoformans. Keywords Mesh Terms:

KEYWORDS: Up-Regulation

MESH TERMS: genetics

Chemical & Substance for Abstract: Cobalt chloride, a hypoxia-mimicking agent, targets sterol synthesis in the pathogenic fungus Cryptococcus neoformans. Information

Substance Name: cobaltous chloride

Registry Number: 7646-79-9

Grant and Affiliation Information for Cobalt chloride, a hypoxia-mimicking agent, targets sterol synthesis in the pathogenic fungus Cryptococcus neoformans.

AFFILIATION: Laboratory of Clinical Infectious Diseases, NIAID, NIH, Bethesda, MD 20892, USA.

Country: England

AGENCY: United States NHLBI

GRANT: HL077588

ACRONYM: HL

MEDLINETA: Mol Microbiol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Cobalt chloride, a hypoxia-mimicking agent, targets sterol synthesis in the pathogenic fungus Cryptococcus neoformans Related Publications

• A FRET-based approach to ratiometric fluorescence detection of hydrogen peroxide.
• A selective fluorescent sensor for detecting lead in living cells.
• A synchrotron radiation beam-position monitor at the Taiwan Light Source.
• Colorimetric identification of carbohydrates by a pH indicator/pH change inducer ensemble.
• Combinatorial synthesis of benzimidazolium dyes and its diversity directed application toward GTP-selective fluorescent chemosensors.
• Complication of an interbody biopolymer graft extruded orally after anterior cervical discectomy and fusion.
• Dedifferentiation? What's next?
• Diagnostic and treatment interventions for hypochondriasis in the neurology setting.
• HIV-1 gp120 up-regulation of the mu opioid receptor in TPA-differentiated HL-60 cells.
• Improvement of generalised dystonia by olanzapine treatment.
• Laparoscopic treatment of colorectal neoplasia.
• Low catheter sepsis rate in a hospital-wide nutrition support service.
• MR imaging of giant intracranial aneurysm.
• Molecular evolution on chiro-inositol dibenzoate using intramolecular acyl migration and selection by phenyl boronic acid.
• Multiple degradation pathways for misfolded mutants of the yeast plasma membrane ATPase, Pma1.
• Native Hawaiian preferences for informed consent and disclosure of results from genetic research.
• Neural control of the release and action of secretin.
• Nutritional assessment using a microcomputer 1. Programme design.
• Overview of prostate-specific membrane antigen.
• Prognostic significance of cytologic features in fine-needle aspiration cytology samples of papillary thyroid carcinoma: preliminary report.
• Propofol hemisuccinate protects neuronal cells from oxidative injury.
• Racial residential segregation and weight status among US adults.
• Regulation of the neurofibromatosis 2 gene promoter expression during embryonic development.
• Silencing of p29 affects DNA damage responses with UV irradiation.
• The commissioning of a flexible low-cost multipurpose X-ray beamline at SRRC.
• Vascular biology and stroke.
• Visual artificial tongue for quantitative metal-cation analysis by an off-the-shelf dye array.
• [Determination of beryllium in geological samples with slurry sampling and probe atomization in graphite furnace atomic absorption spectrometry]
• [Different expression of chaperone interacting protein in normal, scar and chronic ulcer tissues and its relationship with wound healing]
• [Study on the method for the determination of nitric nitrogen, ammoniacal nitrogen and total nitrogen in plant]