Co-lyophilization with D-proline greatly enhances peroxidase's stereoselectivity in a non-aqueous medium.

Co-lyophilization with D-proline greatly enhances peroxidase's stereoselectivity in a non-aqueous medium. Research Abstract Details 

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Co-lyophilization with D-proline greatly enhances peroxidase's stereoselectivity in a non-aqueous medium. Abstract Text:

Ju-Hyun Yu,Alexander M Klibanov,

The impact of co-lyophilizing horseradish peroxidase (HRP) with numerous amino acids and other chiral excipients on the enzyme's subsequent stereoselectivity [E(S/R)] in the sulfoxidation of thioanisole in 2-propanol was systematically investigated. While many improved the stereoselectivity of (and significantly activated) HRP, the greatest effect was observed with D-proline which enhanced the E(S/R) value by over an order of magnitude from synthetically meaningless to useful.

Co-lyophilization with D-proline greatly enhances peroxidase's stereoselectivity in a non-aqueous medium. Publishing Authors By Initials

JH Yu,AM Klibanov,

For similar inorganic chemicals: electrolytes: ions: anions: sulfides research abstracts see: inorganic chemicals: electrolytes: ions: anions: sulfides research

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Co-lyophilization with D-proline greatly enhances peroxidase's stereoselectivity in a non-aqueous medium. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biotechnology letters

VOLUME: 28

Page Numbers: 555-8

Journal Abbreviation: Biotechnol. Lett.

ISSN: 0141-5492

DAY: 3

MONTH: Apr

YEAR: 2006

Co-lyophilization with D-proline greatly enhances peroxidase's stereoselectivity in a non-aqueous medium. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8008051

Co-lyophilization with D-proline greatly enhances peroxidase's stereoselectivity in a non-aqueous medium. Keywords Mesh Terms:

KEYWORDS: Sulfides

MESH TERMS: metabolism

Chemical & Substance for Abstract: Co-lyophilization with D-proline greatly enhances peroxidase's stereoselectivity in a non-aqueous medium. Information

Substance Name: Horseradish Peroxidase

Registry Number: EC 1.11.1.-

Grant and Affiliation Information for Co-lyophilization with D-proline greatly enhances peroxidase's stereoselectivity in a non-aqueous medium.

AFFILIATION: Department of Chemistry and Division of Biological Engineering, Massachusetts Institute of Technology, Cambridge, 02139, USA.

Country: Netherlands

AGENCY: United States NIGMS

GRANT: R01-GM66712

ACRONYM: GM

MEDLINETA: Biotechnol Lett

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