[Co-expression of human bone morphogenetic protein-2 and osteoprotegerin in myoblast C2C12]

[Co-expression of human bone morphogenetic protein-2 and osteoprotegerin in myoblast C2C12] Research Abstract Details 

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[Co-expression of human bone morphogenetic protein-2 and osteoprotegerin in myoblast C2C12] Abstract Text:

Ji-zhong Liu,Yun-yu Hu,Zong-ling Ji,

OBJECTIVE: To construct a co-expressing vector of human bone morphogenetic protein 2 (BMP-2) and osteoprotegerin (OPG) and to determine the expression of BMP-2 and OPG in myoblast C2C12. METHODS: Using the isolated total RNA from osteosacoma cell line MG63 as a template, the cDNA encoding region of human OPG was amplified by reverse transcription-polymerase chain reaction (RT-PCT) method and cloned into sites EcoR 1 and BamH I of mammalian expressing vector pIRES2-EGFP, and the cDNA encoding region of human BMP-2 was cloned into endonucleases site BstX I. Then the recombinant plasmid pIRES2-BMP-2-OPG was transformed into C2C12 cell line, the expression of OPG and BMP-2 were determined by Western blot assay. RESULTS: The sequence of OPG cDNA obtained was the same as that reported, recombinant plasmid pIRES2-BMP-2-OPG was constructed successfully. Human OPG and BMP-2 co-expression cell line C2C12 was selected and confirmed by Western blot analysis. CONCLUSION: The co-expressing vector of OPG and BMP-2 is constructed and can expressed stably in myoblast C2C12. The co-expression of human OPG and BMP-2 may be logical approach for treatment of osteoporosis and bone metastasis.

[Co-expression of human bone morphogenetic protein-2 and osteoprotegerin in myoblast C2C12] Publishing Authors By Initials

JZ Liu,YY Hu,ZL Ji,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research

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[Co-expression of human bone morphogenetic protein-2 and osteoprotegerin in myoblast C2C12] Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Zhongguo xiu fu chong jian wai ke za zhi = Zhonggu

VOLUME: 17

Page Numbers: 1-4

Journal Abbreviation:

ISSN: 1002-1892

DAY: 19

MONTH: Jan

YEAR: 2003

[Co-expression of human bone morphogenetic protein-2 and osteoprotegerin in myoblast C2C12] Information

Number of References:

LANGUAGE: chi

NlmUniqueID: 9425194

[Co-expression of human bone morphogenetic protein-2 and osteoprotegerin in myoblast C2C12] Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta

MESH TERMS: genetics

Chemical & Substance for Abstract: [Co-expression of human bone morphogenetic protein-2 and osteoprotegerin in myoblast C2C12] Information

Substance Name: recombinant human bone morphogenetic pro

Registry Number: 0

Grant and Affiliation Information for [Co-expression of human bone morphogenetic protein-2 and osteoprotegerin in myoblast C2C12]

AFFILIATION: Institute of Orthopedic Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an Shaanxi, P. R. China 710032.

Country: China

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MEDLINETA: Zhongguo Xiu Fu Chong Jian Wai

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