Co-Expression and Purification of Recombinant Human Insulin-Like Growth Factor II and Insulin-Like Growth Factor Binding Protein-6 in Pichia pastoris Yeast.

Co-Expression and Purification of Recombinant Human Insulin-Like Growth Factor II and Insulin-Like Growth Factor Binding Protein-6 in Pichia pastoris Yeast. Research Abstract Details 

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Co-Expression and Purification of Recombinant Human Insulin-Like Growth Factor II and Insulin-Like Growth Factor Binding Protein-6 in Pichia pastoris Yeast. Abstract Text:

Huina Zhou,Zhaoli Chen,Hong Chen,Shuxing Li,Bingren Huang,Ruchang Bi,Huina Zhou,Zhaoli Chen,Hong Chen,Shuxing Li,Bingren Huang,Ruchang Bi,Huina Zhou,Zhaoli Chen,Hong Chen,Shuxing Li,Bingren Huang,Ruchang Bi,

For the preparation of the complex of IGF-II and IGFBP-6, a co-expression vector containing two copies of human IGF-II and IGFBP-6 expression cassette was constructed with alcohol oxidase (AOX1) promoter and secretion signal sequence of alpha-factor, and transformed to Pichia pastoris yeast. Through a purification procedure involving anion-exchange chromatography and gel filtration, a complex of IGF-II with IGFBP-6 was obtained. An additional C-terminal sequence of IGFBP-6 (CS-BP6) was found to be bound to this complex. Dynamic light scattering showed that this complex was very stable and homogenous in solution. Western blotting based on non-reducing Tricine-SDS-PAGE indicated that IGF-II expression coupled with IGFBP-6 might significantly avoid the mispairing of disulfide bonds compared with the IGF-II expressed alone.

Co-Expression and Purification of Recombinant Human Insulin-Like Growth Factor II and Insulin-Like Growth Factor Binding Protein-6 in Pichia pastoris Yeast. Publishing Authors By Initials

H Zhou,Z Chen,H Chen,S Li,B Huang,R Bi,H Zhou,Z Chen,H Chen,S Li,B Huang,R Bi,H Zhou,Z Chen,H Chen,S Li,B Huang,R Bi,

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Co-Expression and Purification of Recombinant Human Insulin-Like Growth Factor II and Insulin-Like Growth Factor Binding Protein-6 in Pichia pastoris Yeast. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Protein and peptide letters

VOLUME: 14

Page Numbers: 876-80

Journal Abbreviation: Protein Pept. Lett.

ISSN: 0929-8665

DAY: 29

MONTH: 11

YEAR: 2007

Co-Expression and Purification of Recombinant Human Insulin-Like Growth Factor II and Insulin-Like Growth Factor Binding Protein-6 in Pichia pastoris Yeast. Information

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LANGUAGE: eng

NlmUniqueID: 9441434

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Grant and Affiliation Information for Co-Expression and Purification of Recombinant Human Insulin-Like Growth Factor II and Insulin-Like Growth Factor Binding Protein-6 in Pichia pastoris Yeast.

AFFILIATION: Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China. huangbr@public.bta.net.cn.

Country: Netherlands

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MEDLINETA: Protein Pept Lett

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