Co-culture of day-5 to day-7 equine embryos in medium with oviductal tissue.

Co-culture of day-5 to day-7 equine embryos in medium with oviductal tissue. Research Abstract Details 

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Co-culture of day-5 to day-7 equine embryos in medium with oviductal tissue. Abstract Text:

D A Freeman,J E Butler,J A Weber,R T Geary,G L Woods,

Oviductal and uterine embryos were collected from mares at 5 to 7 days following ovulation 1) to evaluate the effects of oviductal tissue explants on in vitro growth and development of equine embryos and 2) to study the morphologic development of equine embryos in culture. Embryos were incubated for 5 days in a medium (control group) or in medium supplemented with oviductal tissue explants (co-culture group). Embryos were evaluated and the media changed daily. Following 5 days in culture, 10 10 (100%) control embryos and 27 29 (93%) co-cultured embryos had doubled in diameter. All embryos that were recovered as morulae developed to the blastocyst stage in culture. By 5 days in culture, 6 10 (60%) control embryos and 19 29 (66%) co-cultured embryos had reached the hatching blastocyst stage of development. By 3 days in culture, significantly more (P<0.05) control embryos versus co-cultured embryos had degenerated (4 10 vs 2 29 , respectively). By 5 days in culture, significantly more (P<0.01) control embryos versus co-cultured embryos had degenerated (6 10 vs. 3 29 , respectively). Embryos cultured with oviductal tissue were sustained longer than embryos cultured in medium alone. Hatching was characterized by the blastocyst squeezing through a small opening in the zona pellucida or by the zona pellucida thinning over approximately half of the blastocyst surface and subsequently disappearing entirely.

Co-culture of day-5 to day-7 equine embryos in medium with oviductal tissue. Publishing Authors By Initials

DA Freeman,JE Butler,JA Weber,RT Geary,GL Woods,

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Co-culture of day-5 to day-7 equine embryos in medium with oviductal tissue. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Theriogenology

VOLUME: 36

Page Numbers: 815-22

Journal Abbreviation: Theriogenology

ISSN: 0093-691X

DAY: 26

MONTH: Nov

YEAR: 1991

Co-culture of day-5 to day-7 equine embryos in medium with oviductal tissue. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Co-culture of day-5 to day-7 equine embryos in medium with oviductal tissue.

AFFILIATION: Northwest Equine Reproduction Laboratory Department of Animal and Veterinary Science University of Idaho Moscow, ID 83843 USA.

Country: United States

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MEDLINETA: Theriogenology

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