Clustering of Sp1 sites near the promoter region of ICP34.5 in herpes simplex virus type 1.

Clustering of Sp1 sites near the promoter region of ICP34.5 in herpes simplex virus type 1. Research Abstract Details 

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Clustering of Sp1 sites near the promoter region of ICP34.5 in herpes simplex virus type 1. Abstract Text:

I K Chung,S M Soisson,M T Muller,

We report that a host nuclear protein of approximately 100 kDa binds to the tandemly reiterated DR2 sequence of herpes simplex virus type 1 (HSV-1). The DR2 sequence is a repeated component in the "a" sequence, which defines the signals for cleavage and encapsidation of viral DNA; the "a" sequence also contains the promoter regulatory signals for the gene encoding the viral neurovirulence factor, ICP34.5. Characterization of the host binding protein by means of gel shifts and DNase I footprinting revealed this protein is the eukaryotic transcription factor, Sp1. Furthermore, as judged from the sequence homology, the DR2 region contains clustered matches to the consensus binding site for Sp1. Comparison of the host factor and purified Sp1 (by means of gel shifts and footprinting) confirmed these findings. Since clustered DNA recognition elements represent unusually high affinity binding sites, these repeated Sp1 motifs proximal to the ICP34.5 gene suggest that this region may be a major Sp1 binding site in the viral genome.

Clustering of Sp1 sites near the promoter region of ICP34.5 in herpes simplex virus type 1. Publishing Authors By Initials

IK Chung,SM Soisson,MT Muller,

For similar proteins: viral proteins research abstracts see: proteins: viral proteins research

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Clustering of Sp1 sites near the promoter region of ICP34.5 in herpes simplex virus type 1. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 117

Page Numbers: 19-22

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jan

YEAR: 1995

Clustering of Sp1 sites near the promoter region of ICP34.5 in herpes simplex virus type 1. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Clustering of Sp1 sites near the promoter region of ICP34.5 in herpes simplex virus type 1. Keywords Mesh Terms:

KEYWORDS: Viral Proteins

MESH TERMS: genetics

Chemical & Substance for Abstract: Clustering of Sp1 sites near the promoter region of ICP34.5 in herpes simplex virus type 1. Information

Substance Name: gamma 34.5 protein, Human herpesvirus 1

Registry Number: 0

Grant and Affiliation Information for Clustering of Sp1 sites near the promoter region of ICP34.5 in herpes simplex virus type 1.

AFFILIATION: Department of Biology, College of Science, Yonsei University, Seoul, Korea.

Country: JAPAN

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MEDLINETA: J Biochem

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