[Cloning of the NS1 gene of dengue-2 virus and determination of its partial nucleotide sequence]

[Cloning of the NS1 gene of dengue-2 virus and determination of its partial nucleotide sequence] Research Abstract Details 

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[Cloning of the NS1 gene of dengue-2 virus and determination of its partial nucleotide sequence] Abstract Text:

E Qin,M Yu,P Yang,B Si,P Xu,G Yan,

In this study, RT-PCR product of the NS1 gene of dengue-2 virus isolated in China was inserted into the DNA of T-vector plasmid. DNAs of the positive clones, which were identified by using the blue/white spot and PCR, were digested with enzymes, indicating that the inserted fragments were the same sizes as those of the amplified NS1 genes. The result of the nucleotide sequence determined by dideoxy chain-termination method indicated that 5'-terminal nucleotide sequence of the amplified NS1 fragment was not changed.

[Cloning of the NS1 gene of dengue-2 virus and determination of its partial nucleotide sequence] Publishing Authors By Initials

E Qin,M Yu,P Yang,B Si,P Xu,G Yan,

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[Cloning of the NS1 gene of dengue-2 virus and determination of its partial nucleotide sequence] Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Zhonghua shi yan he lin chuang bing du xue za zhi

VOLUME: 11

Page Numbers: 59-61

Journal Abbreviation: Zhonghua Shi Yan He Lin Chuang

ISSN: 1003-9279

DAY: 28

MONTH: Mar

YEAR: 1997

[Cloning of the NS1 gene of dengue-2 virus and determination of its partial nucleotide sequence] Information

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LANGUAGE: chi

NlmUniqueID: 9602873

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AFFILIATION: Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100850.

Country: China

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MEDLINETA: Zhonghua Shi Yan He Lin Chuang

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