[Cloning of potato invertase inhibitor St-inh cDNA and its expression in E. coli and functional analysis]

[Cloning of potato invertase inhibitor St-inh cDNA and its expression in E. coli and functional analysis] Research Abstract Details 

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[Cloning of potato invertase inhibitor St-inh cDNA and its expression in E. coli and functional analysis] Abstract Text:

Shan Han Cheng,Jun Liu,Bo Tao Song,Cong Hua Xie,

A full length cDNA clone encoding invertase inhibitor was isolated by RT-PCR combined with 5' RACE from potato (S. tuberosum) tubers of cv. JH and designated as St-inh. The encoding region of St-inh is of 663bp encoding a protein of 221 amino acids. The DNA fragment including St-inh cDNA was cloned into the vector pET28a (+) and expressed successfully in E. coli. Co-incubation of the proteins produced by St-inh in E. coli and the invertase extracts from potato tubers of cv. E1, JH and tomato fruits showed that the invertase activities of potato tubers and tomato fruits decreased by 34.3%, 21% and 33.8% respectively. These results indicated that products of St-INH protein had a function of invertase inhibitors. The analysises of the nucleotide and amino acid sequences using BLAST and T-COFFEE demonstrated that St-inh cDNA was of over 95% homologous to Kunitz-type C and there was a typical domain of Kunitz-type protein [L, I, V, M]-X-D-X-[E, D, N, T, Y-[D, G]-[R, K, H, D, E, N, Q]-X-[L, I, V, M]-X(5)-Y-X-[L, I, V, M. Therefore, it was conjectured that St-inh could be a member of Kunitz-type gene family.

[Cloning of potato invertase inhibitor St-inh cDNA and its expression in E. coli and functional analysis] Publishing Authors By Initials

SH Cheng,J Liu,BT Song,CH Xie,

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[Cloning of potato invertase inhibitor St-inh cDNA and its expression in E. coli and functional analysis] Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Shi yan sheng wu xue bao

VOLUME: 37

Page Numbers: 269-75

Journal Abbreviation: Shi Yan Sheng Wu Xue Bao

ISSN: 0001-5334

DAY: 29

MONTH: Aug

YEAR: 2004

[Cloning of potato invertase inhibitor St-inh cDNA and its expression in E. coli and functional analysis] Information

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LANGUAGE: chi

NlmUniqueID: 413570

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Grant and Affiliation Information for [Cloning of potato invertase inhibitor St-inh cDNA and its expression in E. coli and functional analysis]

AFFILIATION: Central China Branch-Centre of Vegetable Improvement, Wuhan 430070.

Country: China

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MEDLINETA: Shi Yan Sheng Wu Xue Bao

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