[Cloning of human testicular carcinoma antigen MAGE-E1 gene and its expression in E.coli]

[Cloning of human testicular carcinoma antigen MAGE-E1 gene and its expression in E.coli] Research Abstract Details 

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[Cloning of human testicular carcinoma antigen MAGE-E1 gene and its expression in E.coli] Abstract Text:

Li Wang,Qing Li,Ai-Lin Guo,Feng Zhang,Bin Guo,Dai-Ming Fan,

AIM: To clone and express the testicular carcinoma antigen MAGE-E1 gene in E.coli. METHODS: The cDNA encoding human MAGE-E1 gene was amplified by RT-PCR from human glioma cell line BT-325, then the MAGE-E1 gene was inserted into plasmid pGEM-T easy. After sequencing, the MAGE-E1 was cloned into the prokaryotic expression vector pGEX-4T-2 to construct the recombinant expression vector pGEX-4T-2-MAGE-E1 which was used to transform E.coli. RESULTS: The expressed product reached the highest level at 5 h after IPTG induction. SDS-PAGE and scanning analysis of gel density indicated that the expressed protein was about M(r) 41 000 and account for 35% of the total bacterial protein. CONCLUSION: The high efficient expression of the MAGE-E1 gene fragment lays the foundation for further preparing antibody against MAGE-E1 protein and the tumor vaccine for glioma immunotherapy.

[Cloning of human testicular carcinoma antigen MAGE-E1 gene and its expression in E.coli] Publishing Authors By Initials

L Wang,Q Li,AL Guo,F Zhang,B Guo,DM Fan,

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[Cloning of human testicular carcinoma antigen MAGE-E1 gene and its expression in E.coli] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese jour

VOLUME: 19

Page Numbers: 148-9

Journal Abbreviation: Xi Bao Yu Fen Zi Mian Yi Xue Z

ISSN: 1007-8738

DAY: 20

MONTH: Mar

YEAR: 2003

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LANGUAGE: chi

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AFFILIATION: Department of Pathology,Xijing Hospital, Fourth Military Medical University, Xi'an 710032,China.

Country: China

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MEDLINETA: Xi Bao Yu Fen Zi Mian Yi Xue Z

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