Cloning, Expression, Purification, Characterization, Crystallization and X-Ray Diffraction of Bifunctional Pyrimidine Deaminase/Reductase from Shigella flexneri 2a.

Cloning, Expression, Purification, Characterization, Crystallization and X-Ray Diffraction of Bifunctional Pyrimidine Deaminase/Reductase from Shigella flexneri 2a. Research Abstract Details 

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Cloning, Expression, Purification, Characterization, Crystallization and X-Ray Diffraction of Bifunctional Pyrimidine Deaminase/Reductase from Shigella flexneri 2a. Abstract Text:

Daopeng Yuan,Qihai Wang,Wei Gao,Fanyi Sheng,Zhanyu Zhang,Qingyu Lu,Huaixing Cang,Ruchang Bi,Daopeng Yuan,Qihai Wang,Wei Gao,Fanyi Sheng,Zhanyu Zhang,Qingyu Lu,Huaixing Cang,Ruchang Bi,Daopeng Yuan,Qihai Wang,Wei Gao,Fanyi Sheng,Zhanyu Zhang,Qingyu Lu,Huaixing Cang,Ruchang Bi,

Bifunctional pyrimidine deaminase/reductase (RibD) plays an important role during riboflavin biosynthesis in many microorganisms. The 40.4 kDa RibD from Shigella flexneri 2a has been cloned, expressed, purified and characterized. Three Crystals of RibD have been obtained by the hanging-drop technique at 291 K using PEG 20k or NaCl as precipitant. The RibD crystal using PEG 20k as precipitant diffracted to 2.5A.

Cloning, Expression, Purification, Characterization, Crystallization and X-Ray Diffraction of Bifunctional Pyrimidine Deaminase/Reductase from Shigella flexneri 2a. Publishing Authors By Initials

D Yuan,Q Wang,W Gao,F Sheng,Z Zhang,Q Lu,H Cang,R Bi,D Yuan,Q Wang,W Gao,F Sheng,Z Zhang,Q Lu,H Cang,R Bi,D Yuan,Q Wang,W Gao,F Sheng,Z Zhang,Q Lu,H Cang,R Bi,

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Cloning, Expression, Purification, Characterization, Crystallization and X-Ray Diffraction of Bifunctional Pyrimidine Deaminase/Reductase from Shigella flexneri 2a. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Protein and peptide letters

VOLUME: 14

Page Numbers: 925-7

Journal Abbreviation: Protein Pept. Lett.

ISSN: 0929-8665

DAY: 29

MONTH: 11

YEAR: 2007

Cloning, Expression, Purification, Characterization, Crystallization and X-Ray Diffraction of Bifunctional Pyrimidine Deaminase/Reductase from Shigella flexneri 2a. Information

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LANGUAGE: eng

NlmUniqueID: 9441434

Cloning, Expression, Purification, Characterization, Crystallization and X-Ray Diffraction of Bifunctional Pyrimidine Deaminase/Reductase from Shigella flexneri 2a. Keywords Mesh Terms:

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Grant and Affiliation Information for Cloning, Expression, Purification, Characterization, Crystallization and X-Ray Diffraction of Bifunctional Pyrimidine Deaminase/Reductase from Shigella flexneri 2a.

AFFILIATION: Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China. w_gao@bjfu.edu.cn.

Country: Netherlands

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MEDLINETA: Protein Pept Lett

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