Cloning, expression and characterization of plasma platelet-activating factor-acetylhydrolase from guinea pig.

Cloning, expression and characterization of plasma platelet-activating factor-acetylhydrolase from guinea pig. Research Abstract Details 

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Cloning, expression and characterization of plasma platelet-activating factor-acetylhydrolase from guinea pig. Abstract Text:

K Karasawa,O Kuge,K Kawasaki,M Nishijima,Y Nakano,M Tomita,K Yokoyama,M Setaka,S Nojima,

In a previous study, we purified PAF-acetylhydrolase, which converts PAF to an inactive metabolite, lysoPAF, from peritoneal fluid of guinea pigs subjected to experimental endotoxin shock and found that this purified enzyme had similar biochemical properties to the plasma enzyme [Karasawa, K., Yato, M., Setaka, M., and Nojima, S. (1994) J. Biochem. 116, 374-379]. In this study, we isolated a homogeneous enzyme preparation from guinea pig plasma using a similar procedure. The molecular mass of this purified enzyme, as determined by SDS-PAGE was 58-63 kDa, larger than that (43 kDa) of the human enzyme. To elucidate the molecular structure of this enzyme and clarify its relationships with PAF-acetylhydrolases of other species, we isolated and sequenced a cDNA encoding this enzyme. Its cDNA contains an open reading frame encoding 436 amino acids and its predicted molecular mass (49 kDa) is lower than that of the native enzyme, suggesting that guinea pig plasma PAF-acetylhydrolase, unlike the human enzyme, is modified post-translationally, perhaps by glycosylation.

Cloning, expression and characterization of plasma platelet-activating factor-acetylhydrolase from guinea pig. Publishing Authors By Initials

K Karasawa,O Kuge,K Kawasaki,M Nishijima,Y Nakano,M Tomita,K Yokoyama,M Setaka,S Nojima,

For similar proteins: recombinant proteins research abstracts see: proteins: recombinant proteins research

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Cloning, expression and characterization of plasma platelet-activating factor-acetylhydrolase from guinea pig. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 120

Page Numbers: 838-44

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Oct

YEAR: 1996

Cloning, expression and characterization of plasma platelet-activating factor-acetylhydrolase from guinea pig. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Cloning, expression and characterization of plasma platelet-activating factor-acetylhydrolase from guinea pig. Keywords Mesh Terms:

KEYWORDS: Recombinant Proteins

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: Cloning, expression and characterization of plasma platelet-activating factor-acetylhydrolase from guinea pig. Information

Substance Name: 1-Alkyl-2-acetylglycerophosphocholine Es

Registry Number: EC 3.1.1.47

Grant and Affiliation Information for Cloning, expression and characterization of plasma platelet-activating factor-acetylhydrolase from guinea pig.

AFFILIATION: Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa.

Country: JAPAN

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MEDLINETA: J Biochem

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ACCESSION NUMBER: D67037

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